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Invitrogen
NOVA1 Polyclonal Antibody
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FIGURE: 1 / 9
NOVA1 Antibody (PA5-95571) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of NOVA1 in U2OS cell using NOVA1 Polyclonal Antibody (Product # PA5-95571). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 2 µg/mL and mouse anti-Beta Tubulin antibody overnight at 4°C. DyLight 488 conjugated goat anti-rabbit IgG and DyLight 594 conjugated goat anti-mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95571
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Nova1 recombinant protein (Position: T416-V509).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human PC-3 whole cell, human A549 whole cell, human U87 whole cell, human THP-1 whole cell, rat brain tissue, mouse brain tissue. IHC: human breast cancer tissue, human colorectal adenocarcinoma tissue, human liver cancer tissue, mouse brain tissue, mouse brain tissue, rat brain tissue, rat brain tissue IHC-F: human placenta tissue. ICC/IF: A549 cell, U2OS cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain protein specifically expressed in the developing ventral brainstem and spinal cord recognized by sera of patients with paraneoplastic opsoclonus and breast cancer; neuro-oncological ventral antigen 1; onconeural ventral antigen 1; paraneoplastic Ri antigen; unnamed protein product; ventral neuron-specific protein 1
Gene Aliases: 9430099M15Rik; G630039L02; Nova-1
UniProt ID: (Human) P51513, (Mouse) Q9JKN6
Entrez Gene ID: (Human) 4857, (Mouse) 664883, (Rat) 298992
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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