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Invitrogen
CDKN2B Polyclonal Antibody
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FIGURE: 1 / 1
CDKN2B Antibody (PA5-96390) in WB
Western blot analysis of CDKN2B in extracts of various cell lines. Samples were incubated with CDKN2B Polyclonal antibody (Product # PA5-96390) using a dilution of 1:800, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-96390
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-138 of human CDKN2B (NP_0049272)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.35 mg/mL
Amount
35 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HeLa, SW620, A-549; Cellular Location: Cytoplasm
Immunogen sequence: MREENKGMPS GGGSDEGLAS AAARGLVEKV RQLLEAGADP NGVNRFGRRA IQVMMMGSAR VAELLLLHGA EPNCADPATL TRPVHDAARE GFLDTLVVLH RAGARLDVRD AWGRLPVDLA EERGHRDVAG YLRTATGD
Target Information
The four known 15-to 19 kDa INK4 proteins (p15INK4b, p16INK4a, p18INK4c andp19INK4d) bind and inhibit CDK4 and CDK6 but not other CDKs, including cyclin E-Cdk2, cyclin A-Cdk2 and cyclin B-Cdk1. Like the three D-type cyclins, the INK4 genes are expressed in distinct tissue-specific patterns, suggesting that they are not strictly redundant. 5 p18INK4c is expressed during G1 to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK inhibitory protein; CDK4B inhibitor; CDKN2B; cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4); cyclin-dependent kinase inhibitor p15; cyclin-dependent kinase inhibitor p15INK4b; cyclin-dependent kinase inhibitor protein; cyclin-dependent kinases 4 and 6 binding protein; MTS-2; multiple tumor suppressor 2; p10; p14-INK4b; p14_CDK inhibitor; p14_INK4B; p15; p15 CDK inhibitor; p15-INK4b; p15_INK4B; p15INK4b; unnamed protein product
Gene Aliases: CDK4I; INK4B; MTS2; P15; p15(INK4b); p15INK4b; TP15
UniProt ID: (Human) P42772, (Mouse) P55271
Entrez Gene ID: (Human) 1030, (Mouse) 12579
cyclin-dependent protein serine/threonine kinase inhibitor activity
protein binding
protein kinase binding
p53 binding
SUMO transferase activity
NF-kappaB binding
ligase inhibitor activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
MDM2/MDM4 family protein binding
disordered domain specific binding
ubiquitin ligase inhibitor activity
transforming growth factor beta receptor signaling pathway
negative regulation of cell proliferation
megakaryocyte differentiation
positive regulation of transforming growth factor beta receptor signaling pathway
cellular response to nutrient
negative regulation of epithelial cell proliferation
regulation of G0 to G1 transition
cellular response to cell-matrix adhesion
cellular senescence
regulation of G1/S transition of mitotic cell cycle
negative regulation of G1/S transition of mitotic cell cycle
spleen development
negative regulation of glial cell proliferation
negative regulation of cell cycle G1/S phase transition
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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