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Invitrogen
RHOT1 Polyclonal Antibody
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FIGURE: 1 / 1
RHOT1 Antibody (PA5-96443) in WB
Western blot analysis of RHOT1 in extracts of various cell lines. Samples were incubated with RHOT1 Polyclonal antibody (Product # PA5-96443) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-96443
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.24 mg/mL
Amount
24 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-549, U-87MG, HeLa, Mouse brain, Rat brain; Cellular Location: Mitochondrion outer membrane, Single-pass type IV membrane protein
Target Information
Mitochondrial GTPase involved in mitochondrial trafficking. RHOT1 is probably involved in control of anterograde transport of mitochondria and their subcellular distribution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MIRO-1; Mitochondrial Rho GTPase; Mitochondrial Rho GTPase 1; Ras homolog gene family member T1
Gene Aliases: 2210403N23Rik; AA415293; AF244542; AI834919; Arht1; C430039G08Rik; Miro1; Rhot1; Rnf135
UniProt ID: (Rat) A1L1L6, (Mouse) Q8BG51
Entrez Gene ID: (Rat) 303351, (Mouse) 59040
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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