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Invitrogen
WIPI2 Polyclonal Antibody
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FIGURE: 1 / 2
WIPI2 Antibody (PA5-96714) in WB
Western blot analysis of WIPI2 in extracts of various cell lines. Samples were incubated with WIPI2 Polyclonal antibody (Product # PA5-96714) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-96714
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 187-436 of human WIPI2 (NP_0570872)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.95 mg/mL
Amount
95 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LAALAFDASG TKLATASEKG TVIRVFSIPE GQKLFEFRRG VKRCVSICSL AFSMDGMFLS ASSNTETVHI FKLETVKEKP PEEPTTWTGY FGKVLMASTS YLPSQVTEMF NQGRAFATVR LPFCGHKNIC SLATIQKIPR LLVGAADGYL YMYNLDPQEG GECALMKQHR LDGSLETTNE ILDSASHDCP LVTQTYGAAA GKGTYVPSSP TRLAYTDDLG AVGGACLEDE ASALRLDEDS EHPPMILRTD; Positive Samples: Jurkat, BT-474; Cellular Location: Cytoplasmic side, Peripheral membrane protein, Preautophagosomal structure membrane
Target Information
WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZP434J154; unnamed protein product; WD40 repeat protein interacting with phosphoinositides 2; WIPI-2; WIPI49-like protein 2; WIPI49-like protein 2 variant; WD-repeat protein; 7-bladed beta-propeller
Gene Aliases: 1110018O08Rik; 2510001I10Rik; ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2
UniProt ID: (Human) Q9Y4P8, (Rat) Q6AY57, (Mouse) Q80W47
Entrez Gene ID: (Human) 26100, (Rat) 288498, (Mouse) 74781
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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