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Invitrogen
Pellino 1 Polyclonal Antibody
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FIGURE: 1 / 1
Pellino 1 Antibody (PA5-96982) in WB
Western blot analysis of Pellino 1 in extracts of Rat brain. Samples were incubated with Pellino 1 Polyclonal antibody (Product # PA5-96982) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-96982
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PELI1 (NP_0657022)
Conjugate
Form
Liquid
Concentration
1.36 mg/mL
Amount
136 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MFSPDQENHP SKAPVKYGEL IVLGYNGSLP NGDRGRRKSR FALFKRPKAN GVKPSTVHIA CTPQAAKAIS NKDQHSISYT LSRAQTVVVE YTHDSNTDMF QIGRSTESPI DFVVTDTVPG SQSNSDTQSV QSTISRFACR IICERNPPFT ARIYAAGFDS SKNIFLGEKA AKWKTSDGQM DGLTTNGVLV MHPRNGFTED SKPGIWREIS VCGNVFSLRE TRSAQQRGKM VEIETNQLQD GSLIDLCGAT; Positive Samples: Rat brain
Target Information
The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-Kappa-B. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-epsilon; and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase pellino homolog 1; pellino 1; pellino homolog 1; pellino1
Entrez Gene ID: (Rat) 305549
protein polyubiquitination
positive regulation of cytokine production
Toll signaling pathway
biological_process
positive regulation of B cell proliferation
positive regulation of protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
response to lipopolysaccharide
positive regulation of toll-like receptor 3 signaling pathway
positive regulation of toll-like receptor 4 signaling pathway
negative regulation of T cell proliferation
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to dsRNA
negative regulation of T cell activation
positive regulation of B cell activation
protein K48-linked ubiquitination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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