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Invitrogen
NCoR2 Polyclonal Antibody
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FIGURE: 1 / 7
NCoR2 Antibody (PA5-97032) in ICC/IF
Immunofluorescence analysis of NCOR2 was performed using 70% confluent log phase PC3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NCoR2 Polyclonal Antibody (Product # PA5-97032) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A327... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-97032
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.85 mg/mL
Amount
85 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HeLa, LO2; Cellular Location: Nucleus
Immunogen sequence: MSGSTQPVAQ TWRATEPRYP PHSLSYPVQI ARTHTDVGLL EYQHHSRDYA SHLSPGSIIQ PQRRRPSLLS EFQPGNERSQ ELHLRPESHS YLPELGKSEM EFIESKRPRL ELL
Target Information
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTG repeat protein 26; mSMRT beta; longer SMRT alpha isoform encoded by GenBank Accession Number AF113001; N-CoR2; nuclear receptor corepressor; silencing mediator for retinoid and thyroid hormone receptors; silencing mediator of retinoic acid and thyroid hormone receptor; silencing mediator of retinoid acid and thyroid hormone receptor; silencing mediator of retinoid and thyroid hormone receptors extended isoform splice variant 1; transcriptional corepressor; SMRTe; T3 receptor-associating factor; thyroid-, retinoic-acid-receptor-associated corepressor; TRAC; unnamed protein product
Gene Aliases: CTG26; N-CoR; N-CoR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC-1; TRAC1
UniProt ID: (Human) Q9Y618, (Mouse) Q9WU42
Entrez Gene ID: (Human) 9612, (Rat) 360801, (Mouse) 20602
chromatin binding
transcription corepressor activity
Notch binding
protein binding
enzyme activator activity
ligand-dependent nuclear receptor binding
glucocorticoid receptor binding
histone deacetylase binding
retinoic acid receptor binding
macromolecular complex binding
retinoid X receptor binding
DNA binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
lactation
regulation of cellular ketone metabolic process
cerebellum development
response to estradiol
estrous cycle
negative regulation of transcription, DNA-templated
negative regulation of transcription initiation from RNA polymerase II promoter
negative regulation of androgen receptor signaling pathway
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
in utero embryonic development
heart morphogenesis
epidermis development
gene expression
telencephalon development
cell proliferation in forebrain
forebrain development
glucose homeostasis
negative regulation of fat cell differentiation
protein stabilization
white fat cell differentiation
Type I pneumocyte differentiation
random inactivation of X chromosome
establishment of skin barrier
positive regulation of protein deacetylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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