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Invitrogen
SMEK1 Polyclonal Antibody
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FIGURE: 1 / 4
SMEK1 Antibody (PA5-97089) in IHC (P)
Immunohistochemistry analysis of SMEK1 in paraffin-embedded human esophageal cancer. Samples were incubated with SMEK1 Polyclonal antibody (Product # PA5-97089) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-97089
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 395-594 of human SMEK1 (NP_0012712101)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.71 mg/mL
Amount
71 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VQTFKGLKLR FEQQRERQDN PKLDSMRSIL RNHRYRRDAR TLEDEEEMWF NTDEDDMEDG EAVVSPSDKT KNDDDIMDPI SKFMERKKLK ESEEKEVLLK TNLSGRQSPS FKLSLSSGTK TNLTSQSSTT NLPGSPGSPG SPGSPGSPGS VPKNTSQTAA ITTKGGLVGL VDYPDDDEDD DEDEDKEDTL PLSKKAKFDS; Positive Samples: HeLa, HepG2, Jurkat, MCF-7, Mouse spleen, Rat brain; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Target Information
Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Serine/threonine-protein phosphatase 4 regulatory subunit 3A; SMEK homolog 1
Gene Aliases: 1110034C04Rik; BC064465; Kiaa2010; mKIAA2010; Pp4r3a; Ppp4r3a; RGD1309059; Smek1
UniProt ID: (Mouse) Q6P2K6
Entrez Gene ID: (Mouse) 68734, (Rat) 314388
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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