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          • Primary Antibodies ›
          • HOOK3 Antibodies

          Invitrogen

          HOOK3 Polyclonal Antibody

          View all (8) HOOK3 antibodies

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          Cite HOOK3 Polyclonal Antibody

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          • Antibody Testing Data (3)
          HOOK3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          HOOK3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          HOOK3 Antibody (PA5-98559) in IHC (P)

          Immunohistochemical analysis of HOOK3 in paraffin embedded human colon cancer using a HOOK3 polyclonal antibody (Product # PA5-98559) at a dilution of 1:300. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detect... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          HOOK3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HOOK3 Antibody in Western Blot (WB)
          HOOK3 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          HOOK3 Polyclonal Antibody

          Product Details

          PA5-98559

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:5,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:500
          -

          ELISA (ELISA)

          Assay-Dependent
          -

          Immunoprecipitation (IP)

          1:200-1:2,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant Human Protein Hook homolog 3 protein (357-455AA)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          4.83 mg/mL

          Amount

          100 µg

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.03% ProClin 300

          Storage conditions

          -20°C or -80°C if preferred

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2813172

          Target Information

          Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: h-hook3; hHK3; hook homolog 3; unnamed protein product

          View more View less

          Gene Aliases: HK3

          View more View less

          UniProt ID: (Human) Q86VS8

          View more View less

          Entrez Gene ID: (Human) 84376

          View more View less

          Function(s)
          protein binding microtubule binding dynactin binding identical protein binding dynein light chain binding dynein intermediate chain binding dynein light intermediate chain binding
          Process(es)
          endosome organization lysosome organization endosome to lysosome transport interkinetic nuclear migration cytoskeleton-dependent intracellular transport cytoplasmic microtubule organization microtubule anchoring at centrosome early endosome to late endosome transport negative regulation of neurogenesis Golgi localization protein localization to centrosome protein transport along microtubule protein localization to perinuclear region of cytoplasm
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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