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Invitrogen
AKAP3 Polyclonal Antibody
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FIGURE: 1 / 5
AKAP3 Antibody (PA5-99257) in IHC (P)
Immunohistochemistry analysis of AKAP3 in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with AKAP3 polyclonal antibody (Product # PA5-99257) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-99257
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AKAP3(Accession O75969), corresponding to amino acid residues G199-R249.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total AKAP3.
Target Information
The type II cAMP-dependent protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins, designated A-kinase anchoring proteins (AKAP). AKAP 3, also known as AKAP 110, FSP95, PRKA3 and SOB1, binds both PKA and PDE4A and functions as a scaffolding protein in spermatozoa to regulate local cAMP concentrations and modulate sperm functions. Expression of AKAP 3 in normal tissues is restricted to the testis, where bicarbonate stimulates tyrosine phosphorylation of AKAP 3, thereby increasing its recruitment of PKA. AKAP-3 also exhibits high expression in patients with epithelial ovarian cancer (EOC). It demonstrates tumor-restricted expression and appears to be associated with worse overall survival, which make AKAP 3 a potential target for antigen-specific immunotherapy in EOC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A kinase (PRKA) anchor protein 3; A-kinase anchor protein 110 kDa; A-kinase anchor protein 3; A-kinase anchor protein, 110kDa; AKAP 110; AKAP-3; Cancer/testis antigen 82; CT82; epididymis luminal protein 159; epididymis secretory sperm binding protein; Fibrous Sheath Protein of 95 kDa; fibrousheathin 1; Fibrousheathin I; Fibrousheathin-1; FSP95; PKA binding protein; PRKA3; protein kinase A binding protein AKAP 110; Protein kinase A-anchoring protein 3; putative fibrous sheath protein; Sperm oocyte-binding protein; sperm oocyte-binding protein 1; unnamed protein product
Gene Aliases: AKAP 110; AKAP110; AKAP3; CT82; FSP95; HEL159; PRKA3; SOB1; SPGF82
UniProt ID: (Human) O75969, (Mouse) O88987
Entrez Gene ID: (Human) 10566, (Mouse) 11642, (Rat) 312720
blastocyst hatching
transmembrane receptor protein serine/threonine kinase signaling pathway
single fertilization
acrosome reaction
protein localization
sperm motility
establishment of protein localization
movement of cell or subcellular component
regulation of protein kinase A signaling
biological_process
phosphorylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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