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Invitrogen

Phospho-p21 (Thr145) Polyclonal Antibody

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Phospho-p21 (Thr145) Polyclonal Antibody

Product Details

PA5-99373

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CDKN1A(Accession P38936), corresponding to amino acid residues around phosphorylated Thr145.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818306

Product Specific Information

Antibody detects endogenous levels of p21 Cip1 only when phosphorylated at Threonine 145.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating Fragment 1; alternate gene name=WAF1; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1A; CDNK1A; Cip1); cyclin kinase inhibitor; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; melanoma differentiation associated protein 6; melanoma differentiation-associated protein; OTTHUMP00000016298; p21Cip1/Waf1; putative DNA synthesis inhibitor; SLC12A9; unnamed protein product; Wild type p53 activated fragment 1 (WAF1); wild type p53 activated fragment-1; wild-type p53-activated fragment 1

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Gene Aliases: CAP20; CDKI; CDKN1; CIP1; MDA-6; mda6; P21; p21CIP1; p21WAF; SDI1; UV96; WAF1

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UniProt ID: (Human) P38936, (Mouse) P39689

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Entrez Gene ID: (Human) 1026, (Rat) 114851, (Mouse) 12575

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Function(s)
protein kinase inhibitor activity cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding protein kinase binding cyclin binding ubiquitin protein ligase binding macromolecular complex binding protein serine/threonine kinase binding protein sequestering activity molecular function activator activity molecular function inhibitor activity
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity in utero embryonic development negative regulation of protein phosphorylation positive regulation of protein phosphorylation protein import into nucleus cellular response to DNA damage stimulus mitotic G2 DNA damage checkpoint Ras protein signal transduction regulation of mitotic cell cycle heart development positive regulation of cell proliferation negative regulation of cell proliferation epidermis development response to xenobiotic stimulus response to UV response to toxic substance response to X-ray regulation of G2/M transition of mitotic cell cycle negative regulation of gene expression keratinocyte differentiation negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator positive regulation of B cell proliferation organ regeneration mitotic G1 DNA damage checkpoint cellular response to amino acid starvation cellular response to heat wound healing tissue regeneration intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death keratinocyte proliferation negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of protein kinase activity negative regulation of growth response to arsenic-containing substance fibroblast proliferation positive regulation of fibroblast proliferation nuclear import response to glucocorticoid response to corticosterone regulation of cell cycle response to hyperoxia intestinal epithelial cell maturation cellular response to cell-matrix adhesion cellular response to ionizing radiation cellular response to gamma radiation cellular response to UV-B signal transduction by p53 class mediator cellular senescence oncogene-induced cell senescence intrinsic apoptotic signaling pathway regulation of cell cycle G1/S phase transition response to aldosterone negative regulation of vascular smooth muscle cell proliferation negative regulation of cardiac muscle tissue regeneration regulation of G1/S transition of mitotic cell cycle negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process negative regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process G1/S transition of mitotic cell cycle positive regulation of DNA replication replicative senescence stress-induced premature senescence
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