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Invitrogen
hnRNP A1 Polyclonal Antibody
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FIGURE: 1 / 6
hnRNP A1 Antibody (PA5-99436) in ICC/IF
Immunofluorescent analysis of hnRNP A1 in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and hnRNP A1 polyclonal antibody (Product # PA5-99436) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-99436
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HNRNPA1(Accession P09651), corresponding to amino acid residues V65-D115.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total hnRNP A1.
Target Information
HnRNP A1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; HDP; helix destabilizing protein; helix-destabilizing protein; heterogeneous nuclear ribonucleoprotein A1B protein; heterogeneous nuclear ribonucleoprotein B2 protein; heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1-like 3; hnRNP core protein A1; hnRNP core protein A1-like 3; hnRNP I; HNRNP-I; HNRNPI; HNRPA1MGC102835; HNRPI; MGC102835; nuclear ribonucleoprotein particle A1 protein; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; putative heterogeneous nuclear ribonucleoprotein A1-like 3; RP11-78J21.1; single-strand DNA-binding protein UP1; single-strand RNA-binding protein; single-strand-binding protein; TIS; topoisomerase-inhibitor suppressed; unnamed protein product
Gene Aliases: ALS19; ALS20; Hdp; HDP-1; hnRNP A1; hnRNP-A1; HNRPA1; HNRPA1L3; IBMPFD3; MPD3; UP 1
UniProt ID: (Human) P09651, (Rat) P04256, (Mouse) P49312
Entrez Gene ID: (Human) 3178, (Rat) 29578, (Mouse) 15382
nucleic acid binding
DNA binding
single-stranded DNA binding
RNA binding
single-stranded RNA binding
mRNA 3'-UTR binding
protein binding
protein domain specific binding
miRNA binding
pre-mRNA binding
identical protein binding
telomeric repeat-containing RNA binding
G-rich strand telomeric DNA binding
mRNA binding
RNA strand annealing activity
DNA/DNA annealing activity
sequence-specific mRNA binding
alternative mRNA splicing, via spliceosome
regulation of alternative mRNA splicing, via spliceosome
mRNA splicing, via spliceosome
RNA splicing
male gonad development
positive regulation of gene expression
RNA metabolic process
lung development
negative regulation of telomere maintenance via telomerase
positive regulation of telomere maintenance via telomerase
response to endoplasmic reticulum stress
cellular response to potassium ion
cellular response to glucose starvation
regulation of RNA splicing
positive regulation of action potential
nuclear export
nuclear import
cellular response to epidermal growth factor stimulus
cellular response to sodium arsenite
RNA export from nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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