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Gibco™

EGF Recombinant Human Protein Solution

Catalog number:  PHG0311L

Related applications:

Mammalian Cell Culture

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Description

EGF is a bioactive protein intended for use in cell culture applications. EGF has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin.

Advantages of using our EGF:

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High-purity, recombinant, bioactive protein
Human EGF is one of our more than 250 available recombinant proteins. To help ensure growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High biological activity
In-house activity testing of recombinant proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant human EGF specific activity is determined by the dose dependent proliferation of mouse BALB/3T3 cells.

Proven compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_001963
Endotoxin Level: < 0.1 ng/µg
Form: Liquid
Indicator Cell: Balb/3T3 cells
Mass: 100 µg
Product Line: Gibco®
Protein Form: Recombinant, Ligand
Purity or Quality Grade: Carrier-Free
Reactivity: Human
Research Category: Stem Cell Research, Oncology
Shipping Condition: Dry Ice
Species: Human
Specific Activity: 1.0 x 107 - 2.5 x 106 units⁄mg
Gene ID (Entrez): EGF
Molecular Weight: 6.2 kDa
Purification Method: Sequential Chromatography
Purity: 95 %
Protein Subtype: EGF (Epidermal Growth Factor)
Gene Alias: EGF
Validated Application: Cell Culture
Protein Family: Growth Factors & Receptors
Expression System: E. coli
Biological Activity: ED50 range = 0.1-0.4 ng⁄mL, ED50 range = 0.1-0.4 ng⁄mL
Protein Tag: Untagged

Contents & storage

Store at ≤ 20°C. Upon initial thawing, we recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. The contents should then be apportioned into working aliquots and stored at ≤ 20°C. Avoid repeated freeze⁄thaw cycles. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.