Gibco™

Heat Stable Recombinant Human bFGF

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Description

Heat Stable Recombinant Human bFGF (also known as fibroblast growth factor-basic, FGF-basic, FGF-β, or FGF2) is a full-length protein (containing 155 amino acid residues) that has been engineered for greater stability in cell culture conditions. It sustains ≥80% activity at 37°C for at least 72 hours compared to native bFGF, which has a half-life of <8 hours at 37°C.

Superior performance—retains bioactivity without the use of artificially high concentrations
Improved efficiency—the stable bioactivity of Heat Stable bFGF means you won’t have to supplement with additional bFGF (a necessary step when using native bFGF), and you’ll save money in bFGF reagent costs over the longer term
Easy to use—direct replacement for your current bFGF, allowing for easy substitution in protocols
Advancement without compromise—>90% sequence homology to native bFGF, leaving heparin and FGF receptor binding sites untouched

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Purity or Quality Grade: Carrier-Free
Quantity: 5 μg
Shipping Condition: Approved for shipment on Wet or Dry Ice
Expression System: E. coli
Protein Family: Growth Factors & Receptors
Research Category: Neurobiology, Oncology, Stem Cell Research, Microbiology, Differentiation, Angiogenesis
Species: Human
Validated Application: Cell Culture
Molecular Weight: 19 kDa
Purity: > 95% by SDS-PAGE
Protein Length: 155 aa + 20 aa for N-terminal tag for purification purposes
Biological Activity: ED50 range = 0.6-1.1 ng⁄mL
Sterility: Filtered before lyophilization through a 0.22 micron sterile filter.

Contents & storage

Store the lyophilized protein at −20°C, preferably desiccated.