Gibco™

Heat Stable Recombinant Human bFGF

Catalog number:  PHG0367

 Related applications:

Mammalian Cell Culture

| Primary Cell Culture

| Stem Cell Culture

| Stem Cell Differentiation

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Description

Heat Stable Recombinant Human bFGF (also known as fibroblast growth factor-basic, FGF-basic, FGF-β, or FGF2) is a full-length protein (containing 155 amino acid residues) that has been engineered for greater stability in cell culture conditions. It sustains ≥80% activity at 37°C for at least 72 hours compared to native bFGF, which has a half-life of <8 hours at 37°C.

Sequence identity
Heat Stable Recombinant Human bFGF has comparable biological function compared to native bFGF. It has >90% sequence homology to native bFGF, leaving heparin and FGF receptor binding sites untouched.

Consistency
Heat Stable Recombinant Human bFGF retains bioactivity without the use of artificially high concentrations. Minimal fluctuation in protein activity more closely mimics physiological conditions for cells. This is key for challenging applications such as culturing bFGF-dependent cells or 3D cell culture where medium changes can be tedious.

Easy to use
Heat Stable Recombinant Human bFGF is a direct replacement for your current human bFGF. Reconstitute lyophilized protein in sterile distilled water (refer to product data sheet for recommended concentration). The 5-µg size is ideal for single use in 500 mL of medium at 10 ng/mL working concentration.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Angiogenesis, Differentiation, Microbiology, Neurobiology, Oncology, Stem Cell Research
Biological Activity: ED50 range = 0.6-1.1 ng⁄mL
Endotoxin Level: < 1 EU/µg
Expression: E. coli
Molecular Weight: 19 kDa
Product Size: 5 µg
Protein Family: Growth Factors & Receptors
Protein Length or Portion (a.a.): 155 aa + 20 aa for N-terminal tag for purification purposes
Purity: > 95% by SDS-PAGE
Purity or Quality Grade: Carrier-Free
Species: Human
Validated Application: Cell Culture
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Store the lyophilized protein at −20°C, preferably desiccated.

Documents

Manuals & protocols