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Thermo Scientific™

BRAF [V599E] Recombinant Human Protein

Catalog number:  PV3849

Related applications:

Protein Assays and Analysis

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Description

BRAF acts upstream of MEK in the MAP kinase pathway and is involved in cell division, differentiation, and secretion. The BRAF mutant V599E is oncogenic and found in a variety of cancers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NP_004324.1, V599E
Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical binding/kinetic
Kinase Group: Ser⁄Thr Kinases: TKL Group
Mass: 10 µg
Shipping Condition: Dry Ice
System Compatibility: LanthaScreen® Antibody, LanthaScreen® Substrate, Zin-LYTE™
Tag Position: N-Terminal
Target Entry: BRAF V599E
Target Gene: v-raf murine sarcoma viral oncogene homolog B1
Expression System: Baculovirus
Protein Family: Kinases & Inhibitors
Protein Form: Recombinant, Catalytic
Protein Subtype: Serine⁄Threonine Kinases
Research Category: Oncology, Signal Transduction
Species: Human
Validated Application: Kinase Assay
Gene ID (Entrez): 10488, 673
Molecular Weight: 67.1 kDa
Gene Alias: BRAF V599E
KinaseFamily: RAF Kinase Family
Protein Length: aa 416-766
Protein Tag: GST Tag

Contents & storage

Storage Buffer: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.5mM EDTA, 0.02% Triton X-100, 2mM DTT, 50% glycerol
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.