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      TGFBR2 Recombinant Human Protein

      Catalog number:  PV6123

       Related applications: Protein Assays and Analysis

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      Description

      This recombinant fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
      • Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
      • Expressed in insect cells
      • GST tagged for use with Eu-labeled GST antibody

      Easily Screen for Kinase Binding
      The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

      The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format, with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer’ to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

      TGFBR2 for the LanthaScreen Kinase Binding Assay
      Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).

      This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
      •  Tracer 178 (Cat No. PV5593)
      •  Eu-labeled GST Antibody (Cat No. PV5594)

      For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

      Related Link
      •  Learn more about the LanthaScreen® Eu Binding Assay.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Research Category: Drug Development
      Accession Number: NP_003233
      Form: Frozen
      Gene Aliases: TGFBR2
      Kinase Group: Ser⁄Thr Kinases: TKL Group
      Molecular Weight: 70.3 kDa
      Product Size: 1 mg
      Species: Human
      Validated Application: Kinase Assay
      Protein Tag or Fusion: GST Tag
      Expression: Baculovirus
      Kinase Family: STKR Kinase Family
      Protein Form: Truncated

      Contents & storage

      Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.

      Documents

      Manuals & protocols