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Thermo Scientific™
During checkout, this product will be reported as "Out of Stock" with an estimated availability date. Often, the actual availability date will be earlier. Send inquiries regarding actual availability date to discoverysciences@lifetech.com.
88 more kinases now available to order! See the complete list.
TGFBR2 Recombinant Human Protein
Description
This recombinant fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.• Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
• Expressed in insect cells
• GST tagged for use with Eu-labeled GST antibody
Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.
The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format, with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer’ to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).
TGFBR2 for the LanthaScreen Kinase Binding Assay
Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).
This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
• Tracer 178 (Cat No. PV5593)
• Eu-labeled GST Antibody (Cat No. PV5594)
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Link
• Learn more about the LanthaScreen® Eu Binding Assay.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Accession Number: | NP_003233 |
|---|---|
| Kinase Group: | Ser⁄Thr Kinases: TKL Group |
| Expression System: | Baculovirus |
| Form: | Frozen |
| Protein Form: | Truncated |
| Research Category: | Drug Development |
| Species: | Human |
| Validated Application: | Kinase Assay |
| Molecular Weight: | 70.3 kDa |
| Gene Alias: | TGFBR2 |
| KinaseFamily: | STKR Kinase Family |
| Protein Tag: | GST Tag |
Contents & storage
Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
