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Thermo Scientific™

GSK-3A Human Protein

Catalog number: PV6126
Thermo Scientific™

GSK-3A Human Protein

Catalog number: PV6126
Catalog Number
PV6126
Unit Size
10 µg
Price (USD)
450.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 µg
Price (USD)
1,542.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 mg
Price (USD)
6,570.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV612610 µg
450.00
Your Price:
***
PR9188A100 µg
1,542.00
Your Price:
***
PV61271 mg
6,570.00
Your Price:
***
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This recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
 Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
 Expressed in insect cells
GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor® conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

GSK3A for the LanthaScreen Kinase Binding Assay
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)

This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
 Tracer 236 (Cat No. PV5592)
 Eu-labeled GST Antibody (Cat No. PV5594)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NP_063937.2
Assay Entry
Biochemical binding/kinetic, Biochemical phospho-specific activity (no antibody)
Kinase Group
Ser⁄Thr Kinases: CMGC Group
Readout
Kinetic
Shipping Condition
Dry Ice
System Compatibility
LanthaScreen™
Tag Position
N-Terminal
Target Entry
GSK3A
Expression System
Baculovirus
Form
Frozen
Product Line
LanthaScreen™
Protein Family
Kinases & Inhibitors
Protein Form
Full Length
Protein Subtype
Serine⁄Threonine Kinases
Research Category
Signal Transduction
Species
Human
Validated Application
Kinase Assay
Gene ID (Entrez)
2931
Molecular Weight
78.4 kDa
Purification Method
Purified
Purity
95% by SDS-PAGE
Quantity
10 µg
Gene Alias
GSK-3A
KinaseFamily
GSK Kinase Family
Recombinant
Yes
Protein Tag
GST Tag

Contents & Storage

Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.

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Frequently asked questions (FAQs)

Citations & References