For order quantities > 1, multiple lots may be used to fulfill the order. If you require a single lot, please email discoverysciences@lifetech.com to ensure your needs are accommodated

88 more kinases now available to order! See the complete list.

GSK-3A Human Protein

Catalog number: PV6126

 Related applications: Protein Assays and Analysis | Target & Lead Identification & Validation

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

This recombinant fusion protein consists of full-length glycogen synthase kinase 3 alpha (GSK3A) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
 Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
 Expressed in insect cells
GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Simultaneous binding of an Alexa Fluor® conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

GSK3A for the LanthaScreen Kinase Binding Assay
Glycogen synthase kinase 3 alpha (GSK3A) is a multifunctional protein serine/threonine kinase implicated in the control of several regulatory proteins and transcription factors. (GSK3A Alias: DKFZp686D0638)

This insect cell-expressed recombinant fusion protein of full-length GSK3A and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
 Tracer 236 (Cat No. PV5592)
 Eu-labeled GST Antibody (Cat No. PV5594)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Form: Frozen
Gene ID: 2931
Species: Human
Gene Aliases: GSK-3A
Kinase Group: Ser⁄Thr Kinases: CMGC Group
Product Size: 10 µg
Purification: Purified
Protein Family: Kinases & Inhibitors
Protein Subtype: Serine⁄Threonine Kinases
Accession Number: NP_063937.2
Molecular Weight: 78.4 kDa
Research Category: Signal Transduction
System Compatibility: LanthaScreen®
Validated Application: Kinase Assay
Protein Tag or Fusion: GST Tag
Tag Position: N-Terminal
Expression: Baculovirus
Kinase Family: GSK Kinase Family
Protein Form: Full Length
Shipping Condition: Dry Ice

Contents & storage

Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.

Documents

Manuals & protocols