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HCK Human Protein

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Description

This recombinant fusion protein consists of full-length hematopoietic cell kinase (HCK) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen® Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
•  Compatible and validated with the LanthaScreen® Eu Kinase Binding Assay
•  Expressed in insect cells
GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen® Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen® Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor® conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

HCK for the LanthaScreen Kinase Binding Assay
Hematopoietic cell kinase (HCK) is a SRC-related kinase expressed in lymphoid and hematopoietic cells and may play a role in some forms of leukemia. (HCK Alias: DKFZp686D0638)

This insect cell-expressed recombinant fusion protein of full-length ACVR2A and GST tag has been validated in the LanthaScreen® Eu Kinase Binding Assay with these reagents:
 Tracer 236 (Cat No. PV5592)
 Eu-labeled anti-GST (Cat No. PV5594)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen® Eu Binding Assay.

For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Accession Number: NP_002101
Expression: Baculovirus
Form: Frozen
Gene Aliases: HCK
Gene ID: 3055
Kinase Family: SRC Kinase Family
Kinase Group: Tyrosine Kinases
Molecular Weight: 84.8 kDa
Product Line: LanthaScreen®
Product Size: 10 µg
Protein Family: Kinases & Inhibitors
Protein Form: Full Length
Protein Subtype: Cytoplasmic Tyrosine Kinases
Protein Tag or Fusion: GST Tag
Purification: Purified
Readout: Kinetic
Research Category: Signal Transduction
Shipping Condition: Dry Ice
Species: Human
System Compatibility: LanthaScreen®
Tag Position: N-Terminal
Validated Application: Kinase Assay

Contents & storage

Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.