Thermo Scientific™

HCK Human Protein

Catalog number: PV6129
Thermo Scientific™

HCK Human Protein

Catalog number: PV6129
Catalog Number
PV6129
Unit Size
1 mg
Price (USD)
Price: 7,870.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
100 µg
Price (USD)
Price: 1,822.00
Your Price:
Availability
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Quantity
Catalog Number
Unit Size
10 µg
Price (USD)
Price: 542.00
Your Price:
Availability
-
Quantity
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This recombinant fusion protein consists of full-length hematopoietic cell kinase (HCK) and a glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay. No special measures were taken to activate this kinase.
•  Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
•  Expressed in insect cells
GST tagged for use with Eu-labeled GST antibody

Easily Screen for Kinase Binding
The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements, and makes simple the study of type III (allosteric) inhibitors.

The LanthaScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one hour incubation and no development steps. Binding of an Alexa Fluor™ conjugate or “tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET (Fig 1).

HCK for the LanthaScreen Kinase Binding Assay
Hematopoietic cell kinase (HCK) is a SRC-related kinase expressed in lymphoid and hematopoietic cells and may play a role in some forms of leukemia. (HCK Alias: DKFZp686D0638)

This insect cell-expressed recombinant fusion protein of full-length ACVR2A and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with these reagents:
 Tracer 236 (Cat No. PV5592)
 Eu-labeled anti-GST (Cat No. PV5594)

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Learn more about the LanthaScreen™ Eu Binding Assay.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number
NP_002101
Molecular Weight
84.8 kDa
Quantity
1 mg
Gene Alias
HCK
KinaseFamily
SRC Kinase Family
Host Species
Insect
Recombinant
Yes
Protein Tag
GST Tag
Kinase Group
Tyrosine Kinases
Shipping Condition
Dry Ice
Expression System
Baculovirus
Protein Family
Kinases & Inhibitors
Protein Form
Recombinant, Full Length
Form
Liquid
Protein Subtype
Cytoplasmic Tyrosine Kinases
Validated Application
Kinase Assay
Species
Human
Research Category
Drug Discovery: Signal Transduction

Contents & Storage

Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% NP-40, 2 mM DTT and 50% glycerol. Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.