Thermo Scientific™

AMPK (A1/B1/G3) Recombinant Human Protein

Catalog number:  PV6242

Related applications:

Protein Assays and Analysis

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Description

AMPK (A1/B1/G3) is a member of the AMPK family which are heterotrimeric proteins consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPKs are an important energy-sensing enzyme group in the cells that monitor energy status particularly in response to stress. AMPKs regulate fatty acid and cholesterol synthesis by regulating the key rate-limiting enzymes acetyl-CoA carboxylase and hydroxy beta-methylglutaryl-CoA reductase. The gamma subunit is dominantly expressed in skeletal muscle where it may play a key role in the regulation of energy metabolism. Recombinant full-length human AMPK (combination of A1/B1/G3 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tags.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Kinase Group: Serine/Threonine Kinase
Species: Human
Validated Application: Kinase Assay
Molecular Weight: 63.6/38/51 kDa
Protein Length: aa 10-end
Protein Tag: His Tag

Contents & storage

Storage Buffer: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.