CDK2/cyclin A1 Recombinant Human Protein

      CDK2/cyclin A1 Recombinant Human Protein

      Catalog number:  PV6290

       Related applications: Pharma & Biopharma | Protein Assays and Analysis | Target & Lead Identification & Validation

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      Description

      CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition. Recombinant full-length human CDK2 and CyclinA1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

      • Validated in LanthaScreen® Kinase Binding Assay
      • Protocols available for simple use and optimized performance
      • Multiple pack sizes available for convenient use

      Related Product:
      LanthaScreen® Eu Kinase Binding Assay
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Gene Aliases: CDK2: p33(CDK2) CyclinA1: CCNA1
      Kinase Group: Serine/Threonine Kinase
      Molecular Weight: 60.2/78.5 kDa
      Product Size: 100 µg
      Species: Human
      Validated Application: Kinase Assay
      Protein Tag or Fusion: GST Tag

      Contents & storage

      Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

      Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.

      Documents

      Manuals & protocols