Thermo Scientific™

CDK2/cyclin E1 Recombinant Human Protein

Catalog number: PV6295
Thermo Scientific™

CDK2/cyclin E1 Recombinant Human Protein

Catalog number: PV6295
Catalog Number
PV6295
Unit Size
10 µg
Price (USD)
449.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 µg
Price (USD)
1,542.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 mg
Price (USD)
6,910.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV629510 µg
449.00
Your Price:
***
PV6296100 µg
1,542.00
Your Price:
***
PV62971 mg
6,910.00
Your Price:
***
Product Overview
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CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition. Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry
Biochemical binding/kinetic
Kinase Group
Serine/Threonine Kinase
Target Entry
CDK2/cyclin E1
Expression System
Baculovirus
Form
Liquid
Protein Family
Kinases & Inhibitors
Protein Form
Recombinant
Species
Human
Validated Application
Kinase Assay
Molecular Weight
60.2/73.4 kDa
Quantity
10 µg
Gene Alias
CDK2: p33 (CDK2) CyclinE1: CCNE1, CCNE
Recombinant
Yes
Protein Tag
GST Tag

Contents & Storage

Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.

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Citations & References