Thermo Scientific™

FYN A Recombinant Human Protein

Catalog number:  PV6347

Related applications:

Protein Assays and Analysis

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Description

Fyn A (Fyn isoform A) is a member of the SRC tyrosine kinase oncogene family showing high homology to YES1, FGR and SRC. Fyn has been shown to phosphorylate Dab1, an intracellular adaptor protein that interacts with amyloid precursor protein (APP) and apoE receptor 2 (apoEr2). The interaction of Fyn and Dab1 regulates the phosphorylation, trafficking, and processing of APP and apoEr2. Fyn expression has been shown to be significantly increased in Chronic Myelogenous Leukemia (CML). Knockdown of Fyn with shRNA slows leukemia cell growth, inhibits clonogenicity, and leads to increased sensitivity to imatinib. Full-length recombinant human FYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Kinase Group: Cytoplasmic Tyrosine Kinase
Species: Human
Validated Application: Kinase Assay
Molecular Weight: 87.1 kDa
Gene Alias: SLK, SYN, MGC45350
Protein Tag: GST Tag

Contents & storage

Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.