Thermo Scientific™

CDK14 (PFTK1)/cyclin Y Recombinant Human Protein

Catalog number:  PV6382

Related applications:

Protein Assays and Analysis

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Description

CDK14 (PFTK1)/cyclin Y is a member of the CDC2-related protein kinase family which is expressed primarily in the postnatal and adult nervous system. CDK14 is expressed at high levels in brain, pancreas, kidney, heart, testis, and ovary. CDK14 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms, while ser120 may contribute slightly to CDK14 binding. CDK14 acts as a cyclin-dependent kinase that regulates cell cycle progression and cell proliferation. Overexpression of CDK14 predicts resistance to chemotherapy in patients with oesophageal squamous cell carcinoma. Recombinant human full-length CDK14 and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 insect cells using N-terminal GST tags.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Biochemical binding/kinetic
Kinase Group: Serine/Threonine Kinase
Target Entry: CDK14 (PFTK1)/cyclin Y 
Species: Human
Validated Application: Kinase Assay
Molecular Weight: 77.0/65.5 kDa
Protein Length: aa Full, 2-end
Protein Tag: GST Tag

Contents & storage

Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.