PRKACG (PRKAC gamma) Recombinant Human Protein

Catalog number:  PV6391

Related applications:

Protein Biology

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Description

PRKACG (PRKAC gamma) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C-alpha and C-beta and showed tissue-specific expression. Whereas at the amino acid level C-alpha and C-beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-beta. The PRKACG gene is intronless, contains remnants of a poly(A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene. Recombinant full-length human PRKACG was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Biochemical binding/kinetic
Kinase Group: Serine/Threonine Kinase
Target Entry: PRKACG (PRKAC gamma)
Species: Human
Validated Application: Kinase Assay
Molecular Weight: 66.7 kDa
Gene Alias: KAPG, PKAr, cAPKr
Protein Tag: GST Tag

Contents & storage

Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.