ULK1 Recombinant Human Protein

Catalog number: PV6430

ULK1 Recombinant Human Protein

Catalog number: PV6430
Catalog Number
PV6430
Unit Size
10 µg
Price (USD)
Price: 506.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV643010 µg
Price: 506.00
Your Price:
***
Product Overview
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ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner. Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Molecular Weight
96.1 kDa
Quantity
10μg
Gene Alias
ATG1, UNC51, Unc51.1, FLJ38455
Protein Length
aa 1-649
Recombinant
Yes
Protein Tag
GST Tag
Assay Entry
Biochemical binding/kinetic
Kinase Group
Serine/Threonine Kinase
Target Entry
ULK1
Expression System
Baculovirus
Protein Family
Kinases & Inhibitors
Protein Form
Recombinant, Fragment
Form
Liquid
Protein Subtype
Serine/Threonine Kinases
Validated Application
Kinase Assay
Species
Human

Contents & Storage

Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.