ULK1 Recombinant Human Protein

Catalog number: PV6430

ULK1 Recombinant Human Protein

Catalog number: PV6430
Catalog Number
PV6430
Unit Size
10 µg
Price (USD)
449.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
PV643010 µg
449.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner. Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry
Biochemical binding/kinetic
Kinase Group
Serine/Threonine Kinase
Target Entry
ULK1
Expression System
Baculovirus
Form
Liquid
Protein Form
Recombinant
Species
Human
Validated Application
Kinase Assay
Molecular Weight
96.1 kDa
Quantity
10μg
Gene Alias
ATG1, UNC51, Unc51.1, FLJ38455
Protein Length
aa 1-649
Recombinant
Yes
Protein Tag
GST Tag

Contents & Storage

Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.

Figures

Frequently asked questions (FAQs)

Citations & References