ULK3 or unc-51-like kinase 3 is a member of the serine/threonine kinase family that is involved in the SHH signaling pathway as a positive regulator of GLI proteins. ULK3 enhances endogenous and over-expressed GLI transcriptional activity in cultured cells and alters its subcellular localization. Furthermore, ULK3 phosphorylates GLI proteins in vitro. ULK3 is widely expressed and its expression is higher in a number of tissues where SHH signaling is known to be active, again suggesting that ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins. Full-length recombinant human ULK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
• Validated in LanthaScreen® Kinase Binding Assay • Protocols available for simple use and optimized performance • Multiple pack sizes available for convenient use