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SIK1 is a protein kinase that is involved in regulating AMPK-related kinases. This recombinant human SIK1 (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
SIK1 may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK1 is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4, and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells.
Learn more about our kinase proteins >
Related Links:
LanthaScreen™ Kinase Activity Assay
LanthaScreen™ Eu Kinase Binding Assay
• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
SIK1 may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK1 is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4, and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells.
Learn more about our kinase proteins >
Related Links:
LanthaScreen™ Kinase Activity Assay
LanthaScreen™ Eu Kinase Binding Assay
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NP_173354
Gene ID (Entrez)
150094
Molecular Weight
61 kDa
Quantity
100μg
Gene Alias
SNF1LK, MSK, SIK1
Protein Length
aa 1-303
Recombinant
Yes
Protein Tag
GST Tag
Kinase Group
Serine/Threonine Kinase
Expression System
Baculovirus
Protein Family
Kinases & Inhibitors
Protein Form
Recombinant, Catalytic Domain
Form
Liquid
Validated Application
Kinase Assay
Species
Human
Research Category
Drug Development, Signal Transduction
Contents & Storage
Storage Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Store at -68 to -85°C. After first use, aliquot and store to avoid multiple freeze-thaws.
Store at -68 to -85°C. After first use, aliquot and store to avoid multiple freeze-thaws.