Qubit® ssDNA Assay Kit

Catalog number: Q10212

Invitrogen™  Related applications: Nucleic Acid Quantitation

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Description

The Qubit® (previously known as Quant-iT™) ssDNA Assay Kit is designed specifically for use with the Qubit® Fluorometer, but may be used with any fluorometer or fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.

Notes:
1. All Qubit® assay kits can be used with the Qubit® 1.0, Qubit® 2.0 and Qubit® 3.0 Fluorometers.
2. 500 µL thin-walled PCR tubes are required but not included.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): ssDNA
For Use With (Equipment): Qubit® Fluorometer
Quantitation Range: 1-200 ng
Product Size: 1 kit
Number of Reactions: 100 Reactions
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C) and protect from light.

Note: 500 µL thin-walled PCR tubes are required but not included.

Documents

Manuals & protocols

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