Imaging tuberculosis with endogenous beta-lactamase reporter enzyme fluorescence in live mice.
AuthorsKong Y, Yao H, Ren H, Subbian S, Cirillo SL, Sacchettini JC, Rao J, Cirillo JD,
JournalProc Natl Acad Sci U S A
PubMed ID20566877
'The slow growth rate and genetic intractability of tubercle bacilli has hindered progress toward understanding tuberculosis, one of the most frequent causes of death worldwide. We overcame this roadblock through development of near-infrared (NIR) fluorogenic substrates for beta-lactamase, an enzyme expressed by tubercle bacilli, but not by their eukaryotic hosts, ... More
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging.
AuthorsMaxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D,
JournalBioconjug Chem
PubMed ID19331388
'Apoptosis is required for normal cellular homeostasis, and deregulation of the apoptotic process is implicated in various diseases. Previously, we developed a cell-penetrating near-infrared fluorescence (NIRF) probe based on an activatable strategy to detect apoptosis-associated caspase activity in vivo. This probe consisted of a cell-penetrating Tat peptide conjugated to an ... More
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin
AuthorsOgawa M, Kosaka N, Choyke PL, Kobayashi H,
JournalBioconjug Chem
PubMed ID19072537
In vivo molecular cancer imaging with monoclonal antibodies has great potential not only for cancer detection, but also for cancer characterization. However, the prolonged retention of intravenously injected antibody in the blood causes low target tumor-to-background ratio (TBR). Avidin has been used as a ... More
Noninvasive optical imaging of cysteine protease activity using fluorescently quenched activity-based probes.
AuthorsBlum G, von Degenfeld G, Merchant MJ, Blau HM, Bogyo M,
JournalNat Chem Biol
PubMed ID17828252
We have generated a series of quenched near-infrared fluorescent activity-based probes (qNIRF-ABPs) that covalently target the papain-family cysteine proteases shown previously to be important in multiple stages of tumorigenesis. These 'smart' probes emit a fluorescent signal only after covalently modifying a specific protease target. After intravenous injection of NIRF-ABPs into ... More
Time-resolved Förster-resonance-energy-transfer DNA assay on an active CMOS microarray.
AuthorsSchwartz DE, Gong P, Shepard KL,
JournalBiosens Bioelectron
PubMed ID18515059
We present an active oligonucleotide microarray platform for time-resolved Förster-resonance-energy-transfer (TR-FRET) assays. In these assays, immobilized probe is labeled with a donor fluorophore and analyte target is labeled with a fluorescence quencher. Changes in the fluorescence decay lifetime of the donor are measured to determine the extent of hybridization. In ... More
Targeted delivery of antigen processing inhibitors to antigen presenting cells via mannose receptors.
AuthorsRaiber EA, Tulone C, Zhang Y, Martinez-Pomares L, Steed E, Sponaas AM, Langhorne J, Noursadeghi M, Chain BM, Tabor AB,
JournalACS Chem Biol
PubMed ID20349916
Improved chemical inhibitors are required to dissect the role of specific antigen processing enzymes and to complement genetic models. In this study we explore the in vitro and in vivo properties of a novel class of targeted inhibitor of aspartic proteinases, in which pepstatin is coupled to mannosylated albumin (MPC6), ... More