Detection of microRNAs in DNA Extractions for Forensic Biological Source Identification.
AuthorsLewis CA, Layne TR, Seashols-Williams SJ
JournalJ Forensic Sci
PubMed ID31107550
'Molecular-based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extraction, requiring an additional step in ... More
Defining quantification methods and optimizing protocols for microarray hybridization of circulating microRNAs.
AuthorsGarcia-Elias A, Alloza L, Puigdecanet E, Nonell L, Tajes M, Curado J, Enjuanes C, Díaz O, Bruguera J, Martí-Almor J, Comín-Colet J, Benito B
JournalSci Rep
PubMed ID28798363
MicroRNAs (miRNAs) have emerged as promising biomarkers of disease. Their potential use in clinical practice requires standardized protocols with very low miRNA concentrations, particularly in plasma samples. Here we tested the most appropriate method for miRNA quantification and validated the performance of a hybridization platform using lower amounts of starting ... More
Circulating miRNAs, isomiRs and small RNA clusters in human plasma and breast milk.
AuthorsRubio M, Bustamante M, Hernandez-Ferrer C, Fernandez-Orth D, Pantano L, Sarria Y, Piqué-Borras M, Vellve K, Agramunt S, Carreras R, Estivill X, Gonzalez JR, Mayor A
JournalPLoS One
PubMed ID29505615
Circulating small RNAs, including miRNAs but also isomiRs and other RNA species, have the potential to be used as non-invasive biomarkers for communicable and non-communicable diseases. This study aims to characterize and compare small RNA profiles in human biofluids. For this purpose, RNA was extracted from plasma and breast milk ... More
Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal.
microRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential. However, our understanding of their usefulness is dependent on the tools we have to study them. Previous studies have identified the need ... More
Differences in microRNA expression between melanoma and healthy adjacent skin.
AuthorsAksenenko M, Palkina N, Komina A, Tashireva L, Ruksha T
JournalBMC Dermatol
PubMed ID30611259
The tumor microenvironment is composed of cancer-associated fibroblasts, tumor-associated macrophages, endothelial cells, immune cells, signaling molecules and extracellular matrix structures, which closelycommunicate with the tumor via multiple mechanisms. MicroRNAs are paracrine regulators that provide a direct interaction between the microenvironment and cancer cells. In the presentstudy, we aimed to identify ... More
Total circulating cell-free miRNA in plasma as a predictive biomarker of the thyroid diseases.
AuthorsCaglar O, Cayir A
JournalJ Cell Biochem
PubMed ID30506793
Thyroid cancer is a common endocrine cancer. Great progress has been made in resolving its molecular mechanisms in recent years. The molecular changes observed in thyroid cancer can be used as biomarkers for diagnostic, prognostic and therapeutic purposes. MicroRNAs (miRNAs) are important components in biological and metabolic pathways, such as ... More
Comparison of methods for miRNA isolation and quantification from ovine plasma.
AuthorsWright K, de Silva K, Purdie AC, Plain KM
JournalSci Rep
PubMed ID31964966
microRNA (miRNA) are promising candidates for disease biomarkers as they are abundant in circulation, highly stable in biological fluids and may yield diagnostic biomarker signatures. The reported issues with miRNA isolation using traditional RNA reagents necessitates the optimisation of miRNA isolation from challenging samples. In this study we compared six ... More