Membrane-based, dry-reagent prothrombin time tests.
AuthorsZweig SE, Meyer BG, Sharma S, Min C, Krakower JM, Shohet SB
JournalBiomed Instrum Technol
PubMed ID8739001
The authors describe a prototype membrane-based, dry-reagent prothrombin time assay for whole blood. This system uses an asymmetric polysulfone membrane to separate plasma from red blood cells, and works with samples as small as 10 microliters. The membrane contains calcium and thromboplastin, and permits the reactions of the complete extrinsic ... More
A homogeneous, nonradioactive high-throughput fluorogenic protein kinase assay.
AuthorsKupcho K, Somberg R, Bulleit B, Goueli SA
JournalAnal Biochem
PubMed ID12758259
Protein kinases play an important role in many cellular processes and mediate cellular responses to a variety of extracellular stimuli. They have been identified by many pharmaceuticals as valid targets for drug discovery. Because of the large number of protein kinases, and the large number of compounds to be screened, ... More
A homogeneous, nonradioactive high-throughput fluorogenic protein phosphatase assay.
AuthorsKupcho K, Hsiao K, Bulleit B, Goueli SA
JournalJ Biomol Screen
PubMed ID15140384
Protein phosphatases are critical components in cellular regulation; they do not only act as antioncogenes by antagonizing protein kinases, but they also play a positive regulatory role in a variety of cellular processes that require dephosphorylation. Thus, assessing the function of these enzymes necessitates the need for a robust, sensitive ... More
Nanoliter homogenous ultra-high throughput screening microarray for lead discoveries and IC50 profiling.
AuthorsMa H, Horiuchi KY, Wang Y, Kucharewicz SA, Diamond SL
JournalAssay Drug Dev Technol
PubMed ID15871692
Microfluidic technologies offer the potential for highly productive and low-cost ultra-high throughput screening and high throughput selectivity profiling. Such technologies need to provide the flexibility of plate-based assays as well as be less expensive to operate. Presented here is a unique microarray system (the Reaction Biology [Malvern, PA] DiscoveryDot), which ... More
Complement component factor B has thrombin-like activity.
Authors
JournalBiochem Biophys Res Commun
PubMed ID33740660
Recombinant chimeric lectins consisting of mannose-binding lectin and L-ficolin are potent inhibitors of influenza A virus compared with mannose-binding lectin.
Authors
JournalBiochem Pharmacol
PubMed ID21035429
Mannose-binding lectin and its associated proteases (MASPs) mediate coagulation and its deficiency is a risk factor in developing complications from infection, including disseminated intravascular coagulation.
Authors
JournalImmunobiology
PubMed ID20399528
Lack of the pattern recognition molecule mannose-binding lectin increases susceptibility to influenza A virus infection.
Authors
JournalBMC Immunol
PubMed ID21182784
Complement 3 is involved with ventilator-induced lung injury.