The SHOX gene encodes for a transcription factor important for normal bone development. Mutations in the gene are associated with idiopathic short stature and are responsible for the growth failure and skeletal defects found in the majority of patients with Léri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia. SHOX is expressed ... More
Disruption of mitochondrial quality control genes promotes caspase-resistant cell survival following apoptotic stimuli.
Authors
JournalJ Biol Chem
PubMed ID35304098
Design and synthesis of novel 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4-thiadiazol- 2-yl)urea derivatives with potent anti-CML activity throughout PI3K/AKT signaling pathway.
AuthorsLi W, Chu J, Fan T, Zhang W, Yao M, Ning Z, Wang M, Sun J, Zhao X, Wen A
JournalBioorg Med Chem Lett
PubMed ID31097376
'In this investigation, a series of 1-phenyl-3-(5-(pyrimidin-4-ylthio)-1,3,4- thiadiazol-2-yl)urea receptor tyrosine kinase inhibitors were synthesized by a simple and efficient structure-based design. Structure-activity relationship (SAR) analysis of these compounds based on cellular assays led to the discovery of a number of compounds that showed potent activity against human chronic myeloid leukemia ... More
SPARC overexpression suppresses radiation-induced HSP27 and induces the collapse of mitochondrial ?? in neuroblastoma cells.
AuthorsTanpure S, Boyineini J, Gnanamony M, Antony R, Fernández KS, Libes J, Lin J, Pinson D, Joseph PA, Gondi CS
JournalOncol Lett
PubMed ID28599461
'Neuroblastoma is the cause of >15% of cancer-associated mortality in children in the USA. Despite aggressive treatment regimens, the long-term survival rate for these children remains at <40%. The current study demonstrates that secreted protein acidic and rich in cysteine (SPARC) suppresses radiation-induced expression of heat shock protein 27 (HSP27)' ... More
The Development of a Nano-based Approach to Alleviate Cisplatin-Induced Ototoxicity.
AuthorsKayyali MN, Ramsey AJ, Higbee-Dempsey EM, Yan L, O'Malley BW, Tsourkas A, Li D
JournalJ Assoc Res Otolaryngol
PubMed ID29349595
'Cisplatin-induced hearing loss is experienced by a high percentage of patients with squamous cell carcinoma undergoing cisplatin chemotherapy. A novel nano-construct capable of sequestering extracellular cisplatin was developed to combat this problem. The nano-construct consisted of superparamagnetic iron oxide nanoparticles (SPIONs) entrapped within polymeric micelles, which were formed from a ... More
Oxidized unsaturated fatty acids induce apoptotic cell death in cultured cells.
AuthorsIuchi K, Ema M, Suzuki M, Yokoyama C, Hisatomi H
JournalMol Med Rep
PubMed ID30720142
'Polyunsaturated fatty acids are oxidized by non-enzymatic or enzymatic reactions. The oxidized products are multifunctional. In this study, we investigated how oxidized fatty acids inhibit cell proliferation in cultured cells. We used polyunsaturated and saturated fatty acids, docosahexaenoic acid (DHA; 22:6), eicosapentaenoic acid (EPA; 20:5), linoleic acid (LA; 18:2), and ... More
Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study.
'Complications of microocclusions have been reported after intra-arterial delivery of mesenchymal stromal cells. Hence, quantification and efficient limitation of cell clumps in suspension before transplantation is important to reduce the risk. We used a flow cytometry-based pulse-width assay to assess the effects of different cell suspension concentrations (0.2-2.0 × 10(6)/mL), ... More
Treatment-Induced Tumor Dormancy through YAP-Mediated Transcriptional Reprogramming of the Apoptotic Pathway.
Authors
JournalCancer Cell
PubMed ID31935369
STING-mediated disruption of calcium homeostasis chronically activates ER stress and primes T cell death.
Authors
JournalJ Exp Med
PubMed ID30886058
Mitotic Vulnerability in Triple-Negative Breast Cancer Associated with LIN9 Is Targetable with BET Inhibitors.
Authors
JournalCancer Res
PubMed ID28807940
Embryonic stem cells become mechanoresponsive upon exit from ground state of pluripotency.
Authors
JournalOpen Biol
PubMed ID30958114
Dissecting the immunosuppressive tumor microenvironments in Glioblastoma-on-a-Chip for optimized PD-1 immunotherapy.
Authors
JournalElife
PubMed ID32909947
Applying genome-wide CRISPR-Cas9 screens for therapeutic discovery in facioscapulohumeral muscular dystrophy.
Authors
JournalSci Transl Med
PubMed ID32213627
An Apoptotic Caspase Network Safeguards Cell Death Induction in Pyroptotic Macrophages.
Authors
JournalCell Rep
PubMed ID32726624
Activation of AMP-activated protein kinase attenuates ethanol-induced ER/oxidative stress and lipid phenotype in human pancreatic acinar cells.
Authors
JournalBiochem Pharmacol
PubMed ID32717227
Abemaciclib Is Effective Against Pancreatic Cancer Cells and Synergizes with HuR and YAP1 Inhibition.
Authors
JournalMol Cancer Res
PubMed ID31383722
Comparison of
AuthorsOpydo-Chanek M, Sladowska K, Blicharski K, Mikeš J, Fedorocko P, Niemeyer U, Mazur L
JournalAnticancer Res
PubMed ID29061820
The oxazaphosphorines, ifosfamide and cyclophosphamide, represent a class of alkylating agents. The aim of the present in vitro study was to compare antileukemic activity of 4-hydroperoxyifosfamide (4-OOH-IF) and 4-hydroperoxycyclophosphamide (4-OOH-CP). ... More
Relaxin confers cytotrophoblast protection from hypoxia-reoxygenation injury through the phosphatidylinositol 3-kinase-Akt/protein kinase B cell survival pathway.
AuthorsOgunleye O, Campo B, Herrera D, Post Uiterweer ED, Conrad KP
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID28122716
Preeclampsia is a hypertensive syndrome that manifests after 20 wk of gestation. Contemporary understanding of the maternal-fetal interface in preeclampsia suggests a major role for placental oxidative stress resulting from ischemia-reperfusion injury. We hypothesized that the pregnancy hormone relaxin would reduce cytotrophoblast apoptosis and necrosis (aponecrosis) and, hence, the export ... More
P-Glycoprotein Inhibition Sensitizes Human Breast Cancer Cells to Proteasome Inhibitors.
AuthorsDeshmukh RR, Kim S, Elghoul Y, Dou QP
JournalJ Cell Biochem
PubMed ID27813130
Although effective for the treatment of hematological malignancies, the FDA approved proteasome inhibitors bortezomib and carfilzomib have limited efficacy in solid tumors including triple negative breast cancer (TNBC). Chemotherapy is the only option for treating TNBC due to the absence of specific therapeutic targets. Therefore, development of new TNBC therapeutic ... More
Role of metabolites of cyclophosphamide in cardiotoxicity.
AuthorsKurauchi K, Nishikawa T, Miyahara E, Okamoto Y, Kawano Y
JournalBMC Res Notes
PubMed ID28807058
The dose-limiting toxic effect of cyclophosphamide (CY) is cardiotoxicity. The pathogenesis of myocardial damage is poorly understood, and there is no established means of prevention. In previous studies, we suggested that for CY-induced cardiotoxicity, whereas acrolein is the key toxic metabolite, carboxyethylphosphoramide mustard (CEPM) is protective. We sought to verify ... More
The Lipophilic Vitamin C Derivative, 6-O-Palmitoylascorbate Protects Human Keratinocytes and 3D-Human Skin Equivalents Against X-Ray-Induced Oxidative Stress and Apoptosis More Markedly Than L-Ascorbic Acid.
AuthorsXiao L, Miwa N
JournalJ Cell Biochem
PubMed ID27350661
The aim of this study was to investigate preventive effects of the lipophilic vitamin C derivative, 6-O-palmitoylascorbate (PlmtVC) against X-ray radiation-induced harmful events. Free radical scavenging activity tests showed that both fresh and old (being kept at 37°C for 72?h) solutions of PlmtVC showed significantly higher abilities for scavenging both ... More
Protective effect of 3-hydroxybutyrate against endoplasmic reticulum stress-associated vascular endothelial cell damage induced by low glucose exposure.
AuthorsSoejima E, Ohki T, Kurita Y, Yuan X, Tanaka K, Kakino S, Hara K, Nakayama H, Tajiri Y, Yamada K
JournalPLoS One
PubMed ID29554103
The aim of this study was to elucidate the mechanism by which severe hypoglycemia accelerates vascular complications. Furthermore, we assessed the possible protective effect of ketone bodies against the endothelial cell damage caused by glucose deficiency. ... More
In vitro leishmanicidal effects of the anti-fungal drug natamycin are mediated through disruption of calcium homeostasis and mitochondrial dysfunction.
AuthorsAwasthi BP, Mitra K
JournalApoptosis
PubMed ID29971703
Natamycin, a Food and Drug Administration approved anti-fungal drug, and also used as a food additive was evaluated for anti-leishmanial activity since it is known to specifically bind to ergosterol, which is essential to these parasites but absent in mammals. Promising anti-proliferative activity was observed in both promastigote and amastigote ... More
Cell-Based High Content Analysis of Cell Proliferation and Apoptosis.
AuthorsMandavilli BS, Yan M, Clarke S
JournalMethods Mol Biol
PubMed ID29082486
High content imaging-based cell cycle analysis allows multiplexing of various parameters including DNA content, DNA synthesis, cell proliferation, and other cell cycle markers such as phosho-histone H3. 5'-Ethynyl-2'-deoxyuridine (EdU) incorporation is a thymidine analog that provides a sensitive method for the detection of DNA synthesis in proliferating cells that is ... More
Abundance of TRAIL attenuated by HIF2a and c-FLIP affects malignancy in renal cell carcinomas.
AuthorsIsono T, Chano T, Yoshida T, Kageyama S, Kawauchi A, Yonese J, Yuasa T
JournalOncotarget
PubMed ID29796174
Dormant cancer cells are starvation-resistant leading to problems in the management of cancer. In renal cell carcinomas (RCCs), starvation-resistant cells are resistant to various currently available therapies. However, targeting hypoxia inducible factor 2-alpha (HIF2-alpha) induces cell death in dormant-like/starvation-resistant RCCs. This study showed that the apoptotic cell death caused by ... More
The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium.
AuthorsLiu W, Ren Z, Lu K, Song C, Cheung ECW, Zhou Z, Chen G
JournalInt J Biol Sci
PubMed ID29805300
Cell density has profound impacts on the cell culture practices of human pluripotent stem cells. The regulation of cell growth, cell death, pluripotency and differentiation converge at high density, but it is largely unknown how different regulatory mechanisms act at this stage. We use a chemically defined medium to systemically ... More
Lipid Analysis of the 6-Hydroxydopamine-Treated SH-SY5Y Cell Model for Parkinson's Disease.
AuthorsXicoy H, Brouwers JF, Kalnytska O, Wieringa B, Martens GJM
JournalMol Neurobiol
PubMed ID31493240
Parkinson's disease (PD) is a highly prevalent neurodegenerative disease for which no disease-modifying treatments are available, mainly because knowledge about its pathogenic mechanism is still incomplete. Recently, a key role for lipids emerged, but lipid profiling of brain samples from human subjects is demanding. Here, we used an unbiased approach, ... More
Application of mild hypothermia successfully mitigates neural injury in a 3D in-vitro model of traumatic brain injury.
AuthorsScimone MT, Cramer HC, Hopkins P, Estrada JB, Franck C
JournalPLoS One
PubMed ID32236105
Therapeutic hypothermia (TH) is an attractive target for mild traumatic brain injury (mTBI) treatment, yet significant gaps in our mechanistic understanding of TH, especially at the cellular level, remain and need to be addressed for significant forward progress to be made. Using a recently-established 3D in-vitro neural hydrogel model for ... More
MAPK14 (p38a) inhibition effects against metastatic gastric cancer cells: A potential biomarker and pharmacological target.
AuthorsMesquita FP, Moreira-Nunes CA, da Silva EL, Lima LB, Daniel JP, Zuerker WJ, Brayner M, de Moraes MEA, Montenegro RC
JournalToxicol In Vitro
PubMed ID32243890
Gastric cancer has been considering one of the worst cancer types since it is diagnosed in advanced stages, currently in the metastatic stage. Therefore, the challenge is to find out a biomarker and a pharmacology target that would help face this worldwide health issue. In this sense, the mitogen-activated protein ... More