Sphingosine-1-phosphate mediates the therapeutic effects of bone marrow mesenchymal stem cell-derived microvesicles on articular cartilage defect.
AuthorsXiang C, Yang K, Liang Z, Wan Y, Cheng Y, Ma D, Zhang H, Hou W, Fu P
JournalTransl Res
PubMed ID29324234
'Microvesicles (MVs) are emerging as a new mechanism of intercellular communication by transferring cellular components to target cells, yet their function in disease is just being explored. However, the therapeutic effects of MVs in cartilage injury and degeneration remain unknown. We found MVs contained high levels of sphingosine-1-phosphate (S1P) compared ... More
The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier.
AuthorsRobilliard LD, Kho DT, Johnson RH, Anchan A, O'Carroll SJ, Graham ES
JournalBiosensors (Basel)
PubMed ID29973526
'In this paper, we demonstrate the application of electrical cell-substrate impedance sensing (ECIS) technology for measuring differences in the formation of a strong and durable endothelial barrier model. In addition, we highlight the capacity of ECIS technology to model the parameters of the physical barrier associated with (I) the paracellular ... More
Diverse Signaling by TGFß Isoforms in Response to Focal Injury is Associated with Either Retinal Regeneration or Reactive Gliosis.
AuthorsConedera FM, Quintela Pousa AM, Presby DM, Mercader N, Enzmann V, Tschopp M
JournalCell Mol Neurobiol
PubMed ID32219603
'Müller cells may have stem cell-like capability as they regenerate photoreceptor loss upon injury in some vertebrates, but not in mammals. Indeed, mammalian Müller cells undergo major cellular and molecular changes summarized as reactive gliosis. Transforming growth factor beta (TGFß) isoforms are multifunctional cytokines that play a central role, both ... More
ECIS technology reveals that monocytes isolated by CD14+ve selection mediate greater loss of BBB integrity than untouched monocytes, which occurs to a greater extent with IL-1ß activated endothelium in comparison to TNFa.
AuthorsKho DT, Johnson R, Robilliard L, du Mez E, McIntosh J, O'Carroll SJ, Angel CE, Graham ES
JournalPLoS One
PubMed ID28732059
We have previously shown that TNFa and IL-1ß differentially regulate the inflammatory phenotype of human brain endothelial cells (hCMVECs). In this regard, IL-1ß treatment was considerably more potent than TNFa at increasing expression of inflammatory chemokines and leukocyte adhesion molecules. We therefore hypothesised that interaction of the hCMVECs with human ... More
Colloid chemistry pitfall for flow cytometric enumeration of viruses in water.
AuthorsDlusskaya EA, Atrazhev AM, Ashbolt NJ
JournalWater Res X
PubMed ID31194069
Flow cytomtery (FCM) has become a standard approach to enumerate viruses in water research. However, the nature of the fluorescent signal in flow cytometric analysis of water samples and the mechanism of its formation, have not been addressed for bacteriophages expected in wastewaters. Here we assess the behaviour of fluorescent ... More
Molecular Mechanism of the Antiproliferative Activity of Short Immunostimulating dsRNA.
AuthorsZharkov MI, Zenkova MA, Vlassov VV, Chernolovskaya EL
JournalFront Oncol
PubMed ID31921696
Small double-stranded RNAs with certain sequence motifs are able to interact with pattern-recognition receptors and activate the innate immune system. Recently, we identified a set of short double-stranded 19-bp RNA molecules with 3-nucleotide 3'-overhangs that exhibited pronounced antiproliferative activity against cancer cells ... More