CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent

Citations & References (7)

Invitrogen™

CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent

製品番号（カタログ番号）	数量
R37167	120 assays

製品番号（カタログ番号） R37167

価格（JPY）

82,300

120 assays

CellEvent™カスパーゼ-3/7 Green Ready Flow™試薬は、アポトーシスを起こしている細胞における活性化されたカスパーゼ-3およびカスパーゼ-7のフローサイトメトリー検出を可能にします。この製品には、新しい蛍光基質CellEvent™カスパーゼ-3/7 Green検出試薬およびSYTOX™ AADvanced™死細胞染色剤の2つの試薬が含まれます。

•室温で安定しています
• 便利なすぐに使えるフォーマット—希釈、重量測定、ピペット操作は不要です
• 核酸への注入時に非常に明るい緑色の蛍光
• 洗浄ステップなしでのアポトーシスを起こしている細胞の迅速な染色

CellEvent Caspase-3/7 Green Ready Flow Reagentは、フローサイトメトリーを使用したカスパーゼ3/7活性を検出する細胞透過性試薬です。ドロッパーボトルから直接1 x 10⁶細胞に2滴を加え、インキュベートして分析します。

CellEvent Caspase-3/7 Green Ready Flow Reagentは、核酸結合色素に結合した4アミノ酸ペプチド（DEVD）で構成されています。アポトーシス中、カスパーゼ-3およびカスパーゼ-7タンパク質が活性化され、DEVDペプチドにエンコードされたカスパーゼ3/7認識配列を開裂できます。認識配列の開裂および試薬によるDNA結合は、アポトーシスを起こしている細胞に約502/530 nmの最大吸収／最大発光を持つ明るい蛍光シグナルを標識します。SYTOX AADvanced Dead Cell Stainと併用すると、アポトーシスを起こしている細胞を生細胞および壊死細胞から簡単に識別できます。

すべてのシステムでアポトーシスを定義する単一パラメータがないため、アポトーシスを確実に検出するために、複数の測定を組み合わせて使用することを強く推奨します。

色Green

励起／発光504／523

標識タイプその他の標識または色素

標識または色素CellEvent™ Caspase 3/7 Green、SYTOX AADvanced死細胞染色

製品ラインCellEvent™ Ready Flow

製品タイプCaspase 3/7試薬

数量120 assays

出荷条件室温

細胞内局在核

フォーマットドロッパーボトル

Unit SizeEach

組成および保存条件

ドロッパーボトル4本、室温で保管してください

よくあるご質問（FAQ）

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?

No, it must be applied to live cell populations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I stain cells with nucleic acid stains?

Yes, you may use SYTOX AADvanced Dead Cell Stain that is included in the CellEvent Caspase-3/7 Green Ready Flow Reagent (Cat. No. R37167) and in the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Cat. No. C10427, C10740). You may also try using other nucleic acid stains in the red or far red channels for staining cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent FAQs

引用および参考文献 (7)

引用および参考文献

Abstract

Alternatives to current flow cytometry data analysis for clinical and research studies.

Authors:Gondhalekar C, Rajwa B, Patsekin V, Ragheb K, Sturgis J, Robinson JP

Journal:Methods

PubMed ID:29305968

'Flow cytometry has well-established methods for data analysis based on traditional data collection techniques. These techniques typically involved manual insertion of tube samples into an instrument that, historically, could only measure 1-3 colors. The field has since evolved to incorporate new technologies for faster and highly automated sample preparation and ... More

Measurement and Characterization of Apoptosis by Flow Cytometry.

Authors:Telford W, Tamul K, Bradford J

Journal:Curr Protoc Cytom

PubMed ID:27367289

'Apoptosis is an important mechanism in cell biology, playing a critical regulatory role in virtually every organ system. It has been particularly well characterized in the immune system, with roles ranging from immature immune cell development and selection to down-regulation of the mature immune response. Apoptosis is also the primary ... More

Computational flow cytometry reveals that cryopreservation induces spermptosis but subpopulations of spermatozoa may experience capacitation-like changes.

Authors:Ortega-Ferrusola C, Anel-López L, Martín-Muñoz P, Ortíz-Rodríguez JM, Gil MC, Alvarez M, de Paz P, Ezquerra LJ, Masot AJ, Redondo E, Anel L, Peña FJ

Journal:Reproduction

PubMed ID:27965398

'The reduced lifespan of cryopreserved spermatozoa in the mare reproductive tract has been attributed to both capacitative and apoptotic changes. However, there is a lack of studies investigating both phenomena simultaneously. In order to improve our knowledge in this particular point, we studied in raw and frozen-thawed samples apoptotic and ... More

Effects of ghrelin on the apoptosis of human neutrophils in vitro.

Authors:Li B, Zeng M, Zheng H, Huang C, He W, Lu G, Li X, Chen Y, Xie R

Journal:Int J Mol Med

PubMed ID:27431014

Acute respiratory distress syndrome (ARDS) is characterized by lung inflammation and the diffuse infiltration of neutrophils into the alveolar space. Neutrophils are abundant, short-lived leukocytes that play a key role in immune defense against microbial infections. These cells die via apoptosis following the activation and uptake of microbes, and will also ... More

New flow cytometry approaches in equine andrology.

Authors:Peña FJ, Ortega Ferrusola C, Martín Muñoz P

Journal:Theriogenology

PubMed ID:27160445

Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the ... More

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