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CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent - FAQs

CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent - FAQs

View additional product information for CellEvent™ Caspase 3/7 Green Ready Flow™ Reagent - FAQs (R37167)

8 product FAQs found

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?

No, it must be applied to live cell populations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I stain cells with nucleic acid stains?

Yes, you may use SYTOX AADvanced Dead Cell Stain that is included in the CellEvent Caspase-3/7 Green Ready Flow Reagent (Cat. No. R37167) and in the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Cat. No. C10427, C10740). You may also try using other nucleic acid stains in the red or far red channels for staining cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With the CellEvent Caspase-3/7 Green Detection Reagent, may I multiplex with fluorescent immunolabeling?

Yes. You can apply surface labels or other reagents. We recommend applying the surface labels or other reagents first.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is the CellEvent Caspase-3/7 Green Detection Reagent fixable?

The CellEvent Caspase-3/7 Green Detection Reagent is not covalently attached to any cellular component and may be lost upon fixation. For flow cytometry applications, after applying the reagent, you should examine without washing or fixation. For imaging applications, the CellEvent product signal may be retained after fixation in 3.7% formaldehdye for 15 min.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I fix my CellEvent Caspase 3/7 - labeled cells?

For imaging applications, we recommend fixing with 3.7% formaldehyde at room temperature for 15 mins. After fixation, cells can be imaged for up to 24 hrs, but the signal will gradually degrade over time. For flow cytometry applications, fixation is not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.