Invitrogen™

Rhodamine Phalloidin

Catalog number: R415
Invitrogen™

Rhodamine Phalloidin

Catalog number: R415
Catalog Number
R415
Unit Size
300 units
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
R415300 units
-
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Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).

Also available as a room-temperature-stable, ready-to-use solution: ActinRed 555 Ready Probes Reagent.
See other Ready Probes ready-to-use imaging reagents and accessories ›

Selectively stains F-actin
Excitiation/Emission: 540/565 nm
Superior to antibody staining
Optimal for fixed and permeabilized samples
Very widely cited fluorescent phalloidin conjugate

Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides 'death cap' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).

Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and Alexa Fluor conjugates including secondary antibodies. For research use only.

Not intended for human or animal therapeutic or diagnostic use.

Related Links
Read about labeling F-Actin with phallotoxins.
Create a Cellular Masterpiece with Alexa Fluor Phalloidin using the Cell Paint Tool.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation Wavelength Range
540⁄565
Dye Type
TRITC (Tetramethylrhodamine Isothiocyanate)
Shipping Condition
Room Temperature
Label Type
Classic Dyes
Product Type
Phalloidin
SubCellular Localization
Actin, Cytoskeleton

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

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