Detection of anti-type 3 muscarinic acetylcholine receptor autoantibodies in the sera of Sjögren's syndrome patients by use of a transfected cell line assay.
AuthorsGao J, Cha S, Jonsson R, Opalko J, Peck AB,
JournalArthritis Rheum
PubMed ID15334476
'OBJECTIVE: Sjögren''s syndrome (SS) is an autoimmune disease affecting primarily the salivary and lacrimal glands, leading to dry mouth and dry eyes. Recent studies have suggested that autoantibodies reactive with the type 3 muscarinic acetylcholine receptors (M3Rs) expressed on salivary and lacrimal gland cells may be highly specific for SS. ... More
The macrophage scavenger receptor CD163: endocytic properties of cytoplasmic tail variants.
AuthorsNielsen MJ, Madsen M, Møller HJ, Moestrup SK,
JournalJ Leukoc Biol
PubMed ID16434690
'CD163 is the monocyte/macrophage-specific receptor for haptoglobin-hemoglobin (Hp-Hb) complexes. The cytoplasmic tail of human CD163 exists as a short tail variant and two long tail variants. Reverse transcriptase-polymerase chain reaction analysis indicated that all three CD163 variants are substantially expressed in blood, liver, and spleen, and the short tail variant ... More
Effect of genetic variation on human cytochrome p450 reductase-mediated paraquat cytotoxicity.
AuthorsHan JF, Wang SL, He XY, Liu CY, Hong JY,
JournalToxicol Sci
PubMed ID16495354
Paraquat (1,1'-dimethyl-4,4'-bipyridylium dichloride) is a widely used herbicide and is highly toxic to human and animals. The mechanisms of paraquat toxicity involve the generation of superoxide anion through the process of redox cycling. NADPH-cytochrome P450 oxidoreductase (POR) has been reported to be a major enzyme for one-electron reduction of paraquat ... More
The missense genetic polymorphisms of human CYP2A13: functional significance in carcinogen activation and identification of a null allelic variant.
AuthorsWang SL, He XY, Shen J, Wang JS, Hong JY,
JournalToxicol Sci
PubMed ID16917071
Cytochrome P450 2A13 (CYP2A13), an enzyme predominantly expressed in human respiratory tissues, is highly efficient for the metabolic activation of two suspected human lung carcinogens 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and aflatoxin B1 (AFB1). Functional genetic polymorphisms of CYP2A13 may therefore be an important factor in human susceptibility to related lung cancers. Among ... More
Targeting of the dual oxidase 2 N-terminal region to the plasma membrane.
Dual oxidase 2 (Duox2) is a cell surface glycoprotein that probably provides thyroperoxidase with the H2O2 required to catalyze thyroid hormone synthesis. No functional H2O2-generating system has yet been obtained after transfecting Duox2 into non-thyroid cell lines, because it is retained in the endoplasmic reticulum (ER). We investigated the level ... More
Enhanced efficiency through nuclear localization signal fusion on phage PhiC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells.
The integrase of the phage PhiC31 recombines an attP site in the phage genome with a chromosomal attB site of its Streptomyces host. We have utilized the integrase-mediated reaction to achieve episomal and genomic deletion of a reporter gene in mammalian cells, and provide the first comparison of its efficiency ... More
Tamapin, a venom peptide from the Indian red scorpion (Mesobuthus tamulus) that targets small conductance Ca2+-activated K+ channels and afterhyperpolarization currents in central neurons.
Authors Pedarzani Paola; D'hoedt Dieter; Doorty Kevina B; Wadsworth Jonathan D F; Joseph Jeremiah S; Jeyaseelan Kandiah; Kini R Manjunatha; Gadre S V; Sapatnekar S M; Stocker Martin; Strong Peter N;
JournalJ Biol Chem
PubMed ID12239213
The biophysical properties of small conductance Ca(2+)-activated K(+) (SK) channels are well suited to underlie afterhyperpolarizations (AHPs) shaping the firing patterns of a conspicuous number of central and peripheral neurons. We have identified a new scorpion toxin (tamapin) that binds to SK channels with high affinity and inhibits SK channel-mediated ... More
Fibulin-5 is an elastin-binding protein essential for elastic fibre development in vivo.
Authors Yanagisawa Hiromi; Davis Elaine C; Starcher Barry C; Ouchi Takashi; Yanagisawa Masashi; Richardson James A; Olson Eric N;
JournalNature
PubMed ID11805834
Extracellular elastic fibres provide mechanical elasticity to tissues and contribute towards the processes of organ remodelling by affecting cell-cell signalling. The formation of elastic fibres requires the assembly and crosslinking of tropoelastin monomers, and organization of the resulting insoluble elastin matrix into functional fibres. The molecules and mechanisms involved in ... More