V5 Tag Monoclonal Antibody (SV5-Pk1)

V5 Tag Monoclonal Antibody (SV5-Pk1) - FAQs

View additional product information for V5 Tag Monoclonal Antibody (SV5-Pk1) - FAQs (R960CUS, R96025)

4 product FAQs found

What is the V5 epitope, and where does it come from?

The V5 epitope is found in the P and V proteins of the Simian paramyxovirus, SV5. The Anti-V5 antibody recognizes the following 14 amino acid sequence: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr

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What applications are appropriate for your Anti-Xpress antibody, Anti-V5 antibody, Anti-HisG antibody, and Anti-myc antibody?

ELISA, Western blot, Immunoprecipitation, Immunofluorescence, and Immunohistochemistry protocols have all been used successfully with our epitope tag antibodies. Please consult the product manuals for specific protocols and dilutions.

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Do you have a recommended protocol for immunoprecipitation (IP) with the Anti-V5 antibody (Cat. No. R96025)?

Yes, we did design a protocol for use of the Anti-V5 antibody in immunoprecipitations, and it is included in the Anti-V5 antibody instruction manual. Search "R96025" to find a copy of the manual on the product detail and ordering page.

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Can the anti-v5 antibody be used for immunostaining?

Yes, the anti-V5 and anti-V5-HRP antibodies can be used in immunohistochemical and immunofluorescent techniques to detect fusion proteins in cells or tissues that contain the -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr- epitope. A protocol is available in the product insert.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.