V5 Tag Monoclonal Antibody (SV5-Pk1) - FAQs

View additional product information for V5 Tag Monoclonal Antibody (SV5-Pk1) - FAQs (R960CUS, R96025)

4 product FAQs found

What is the V5 epitope, and where does it come from?

The V5 epitope is found in the P and V proteins of the Simian paramyxovirus, SV5. The Anti-V5 antibody recognizes the following 14 amino acid sequence: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What applications are appropriate for your Anti-Xpress antibody, Anti-V5 antibody, Anti-HisG antibody, and Anti-myc antibody?

ELISA, Western blot, Immunoprecipitation, Immunofluorescence, and Immunohistochemistry protocols have all been used successfully with our epitope tag antibodies. Please consult the product manuals for specific protocols and dilutions.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you have a recommended protocol for immunoprecipitation (IP) with the Anti-V5 antibody (Cat. No. R96025)?

Yes, we did design a protocol for use of the Anti-V5 antibody in immunoprecipitations, and it is included in the Anti-V5 antibody instruction manual. Search "R96025" to find a copy of the manual on the product detail and ordering page.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can the anti-v5 antibody be used for immunostaining?

Yes, the anti-V5 and anti-V5-HRP antibodies can be used in immunohistochemical and immunofluorescent techniques to detect fusion proteins in cells or tissues that contain the -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr- epitope. A protocol is available in the product insert.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.