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Invitrogen
V5 Tag Monoclonal Antibody (SV5-Pk1)
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FIGURE: 1 / 5
V5 Tag Antibody (R960-25) in ICC/IF
Immunofluorescent analysis of V5 Tag was performed using 70% confluent V5-H3-6XHis transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with V5 Tag Monoclonal Antibody (Product # R960-25) at 1:100 dilution and Histone H3 Recombinant Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse ... View More
Product Details
R960-25
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
SV5-Pk1
Immunogen
V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.
Conjugate
Form
Liquid
Purification
purified
Storage buffer
PBS
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
Product Specific Information
NOTE: R960-25 was previously sold under product #46-0705. When re-ordering, please use #R960-25.
R960CUS was previously sold under product #46-1157. When re-ordering, please use #R960CUS.
This antibody recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.
This antibody is functionally tested against 20 ng of an E. coli expressed fusion protein containing a V5 epitope using a chemiluminescent substrate at a 1 minute exposure. This antibody has also been tested in Western blot against 25 ng of recombinant Positope™ protein. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His (C-term), HisG, c-myc, and V5. Low background was observed using chemiluminescent or alkaline phosphatase reagents for detection. For Western blot, dilute in PBS or Tris-Buffered Saline (TBS) containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM or TBSTM).
Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.
This antibody has also been used successfully to immunoprecipitate fusion proteins that contain theV5 epitope.
Target Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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