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Invitrogen
Human IgG1 Fc (103 Cys/Ser) Recombinant Protein
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Product Details
RP-88063
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human IgG1 Fc, amino acids Glu99-Lys330 (Accession # P01857-1) with a 103 Cys/Ser mutation
Molecular weight
26.08 kDa
Class
Recombinant
Type
Protein
Purity
≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized Recombinant Human IgG1-Fc Protein (103 Cys/Ser) (Cat: 10702-HNAH) at 2µg/mL (100µL/well) can bind
Recombinant Human CD64 / FCGR1A Protein (His Tag) (Cat: 10256-H08H), the EC50 is 1.6-4.8 ng/mL
Recombinant Human CD64 / FCGR1A Protein (His Tag) (Cat: 10256-H08H), the EC50 is 1.6-4.8 ng/mL
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.4 mg/mL once reconstituted
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
Reconstituted with sterile deionized water to 0.4 mg/mL.
Concentration is measured by UV.
Target Information
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: unnamed protein product
Entrez Gene ID: (Human) 3500
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