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Invitrogen
Mouse Angiopoietin-2 His-tag Recombinant Protein
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Product Details
RP-88199
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse ANGPT2, amino acids Lys275-Phe496 (Accession # NP_031452.2) with an N-terminal His-tag
Tag
His-tag
Molecular weight
33.4 kDa
Class
Recombinant
Type
Protein
Purity
>95% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Angiopoietin-2-His at 2 µg/ml (100 µL/well) can bind mouse TEK-Fc, the EC50 of mouse TEK-Fc is 350-900 ng/mL.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Amount
50 µg
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (705 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
ANG-2 binds to the endothelial cell specific receptor Tie-2, but, in contrast to ANG-1, does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie-2 and, depending on the physiological and biochemical environment, can act either as an agonist or antagonist of Tie-2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie-2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha-helix-rich coiled coil N-terminal domain and fibrinogen-like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ligand for the Tie2/Tek receptor tyrosine kinase; unnamed protein product
Gene Aliases: Agpt2; Ang-2; Ang2
UniProt ID: (Mouse) O35608
Entrez Gene ID: (Mouse) 11601
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