View additional product information for SYPRO™ Protein Gel Stains - Citations (S12000, S12001, S6650, S6651, S12010, S12000X3, S6654, S6653, S21900)
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Abstract
The major CD9 and CD81 molecular partner. Identification and characterization of the complexes.
AuthorsCharrin S,Le Naour F,Oualid M,Billard M,Faure G,Hanash SM,Boucheix C,Rubinstein E
JournalThe Journal of biological chemistry
PubMed ID11278880
Autotaxin is released from adipocytes, catalyzes lysophosphatidic acid synthesis, and activates preadipocyte proliferation. Up-regulated expression with adipocyte differentiation and obesity.
Our group has recently demonstrated (Gesta, S., Simon, M., Rey, A., Sibrac, D., Girard, A., Lafontan, M., Valet, P., and Saulnier-Blache, J. S. (2002) J. Lipid Res. 43, 904-910) the presence, in adipocyte conditioned-medium, of a soluble lysophospholipase d-activity (LPLDact) involved in synthesis of the bioactive phospholipid lysophosphatidic acid (LPA). ... More
Changes in global gene and protein expression during early mouse liver carcinogenesis induced by non-genotoxic model carcinogens oxazepam and Wyeth-14,643.
We hypothesized that the mouse liver tumor response to non-genotoxic carcinogens would involve some common early gene and protein expression changes that could ultimately be used to predict chemical hepatocarcinogenesis. In order to identify a panel of genes to test, we analyzed global differences in gene and protein expression in ... More
Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1.
AuthorsGiannattasio M, Sabbioneda S, Minuzzo M, Plevani P, Muzi-Falconi M
JournalJ Biol Chem
PubMed ID12672803
Rad17-Mec3-Ddc1 forms a proliferating cell nuclear antigen-like complex that is required for the DNA damage response in Saccharomyces cerevisiae and acts at an early step of the signal transduction cascade activated by DNA lesions. We used the mec3-dn allele, which causes a dominant negative checkpoint defect in G1 but not ... More
Short-term training enhances endothelium-dependent dilation of coronary arteries, not arterioles.
Our objective was to test the hypothesis that short-term exercise training (STR) of pigs increases endothelium-dependent dilation (EDD) of coronary arteries but not coronary arterioles. Female Yucatan miniature swine ran on a treadmill for 1 h, at 3.5 mph, twice daily for 7 days (STR; n = 28). Skeletal muscle ... More
Identification and characterization of DEN1, a deneddylase of the ULP family.
To identify deneddylases, proteases with specificity for hydrolysis of Nedd8 derivatives, a facile method was developed for the synthesis of Nedd8 amidomethylcoumarin (a substrate) and Nedd8 vinyl sulfone (an inhibitor). Deneddylase activity is necessary to reverse the conjugation of Nedd8 to cullin, a modification that regulates at least some ubiquitin ... More
Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge.
AuthorsOhishi K, Nagamune K, Maeda Y, Kinoshita T
JournalJ Biol Chem
PubMed ID12582175
Many eukaryotic proteins are tethered to the plasma membrane via glycosylphosphatidylinositol (GPI). GPI transamidase is localized in the endoplasmic reticulum and mediates post-translational transfer of preformed GPI to proteins bearing a carboxyl-terminal GPI attachment signal. Mammalian GPI transamidase is a multimeric complex consisting of at least five subunits. Here we ... More
Epididymal lipocalin-type prostaglandin D2 synthase: identification using mass spectrometry, messenger RNA localization, and immunodetection in mouse, rat, hamster, and monkey.
This study identified prostaglandin D2 synthase (PGDS) in murine epididymal fluid using a proteomic approach combining two-dimensional (2D) gel electrophoresis and mass spectrometry (MS). The caudal epididymal fluid was collected by retroperfusion, and proteins were separated by 2D gel electrophoresis followed by matrix-assisted laser desorption ionization MS analyses after trypsin ... More
Purification, characterization, and sequencing of an extracellular cold-active aminopeptidase produced by marine psychrophile Colwellia psychrerythraea strain 34H.
AuthorsHuston AL, Methe B, Deming JW
JournalAppl Environ Microbiol
PubMed ID15184127
The limited database on cold-active extracellular proteases from marine bacteria was expanded by successful purification and initial biochemical and structural characterization of a family M1 aminopeptidase (designated ColAP) produced by the marine psychrophile Colwellia psychrerythraea strain 34H. The 71-kDa enzyme displayed a low optimum temperature (19 degrees C) and narrow ... More
Genome-wide expression analyses of Campylobacter jejuni NCTC11168 reveals coordinate regulation of motility and virulence by flhA.
AuthorsCarrillo CD, Taboada E, Nash JH, Lanthier P, Kelly J, Lau PC, Verhulp R, Mykytczuk O, Sy J, Findlay WA, Amoako K, Gomis S, Willson P, Austin JW, Potter A, Babiuk L, Allan B, Szymanski CM
JournalJ Biol Chem
PubMed ID14985343
We examined two variants of the genome-sequenced strain, Campylobacter jejuni NCTC11168, which show marked differences in their virulence properties including colonization of poultry, invasion of Caco-2 cells, and motility. Transcript profiles obtained from whole genome DNA microarrays and proteome analyses demonstrated that these differences are reflected in late flagellar structural ... More
M line-deficient titin causes cardiac lethality through impaired maturation of the sarcomere.
AuthorsWeinert S, Bergmann N, Luo X, Erdmann B, Gotthardt M
JournalJ Cell Biol
PubMed ID16702235
'Titin, the largest protein known to date, has been linked to sarcomere assembly and function through its elastic adaptor and signaling domains. Titin''s M-line region contains a unique kinase domain that has been proposed to regulate sarcomere assembly via its substrate titin cap (T-cap). In this study, we use a ... More
Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine methyltransferase 3).
AuthorsSwiercz R, Person MD, Bedford MT
JournalBiochem J
PubMed ID15473865
'PRMT3 (protein arginine methyltransferase 3) is one of four type I arginine methyltransferases that catalyse the formation of asymmetric dimethylarginine. PRMT3 is unique in that its N-terminus harbours a C2H2 zinc-finger domain that is proposed to confer substrate specificity. In addition, PRMT3 is the only type I enzyme that is ... More
Quantitative evaluation of sample application methods for semipreparative separations of basic proteins by two-dimensional gel electrophoresis.
AuthorsBarry RC, Alsaker BL, Robison-Cox JF, Dratz EA
JournalElectrophoresis
PubMed ID14595686
'The use of cup-loading for sample application has become widely used in two-dimensional electrophoresis (2-DE) for resolution of basic proteins, but no side-by-side quantitative study has been published which compares cup-loading with the alternative passive and active rehydration methods to fully promote one type of loading method over another. Replicate ... More
Initial analysis of the phosphoproteome of Chinese hamster ovary cells using electrophoresis.
AuthorsChen Z, Southwick K, Thulin CD
JournalJ Biomol Tech
PubMed ID15585821
'Protein phosphorylation is a common post-translational modification of enormous biological importance. Analysis of phosphorylation at the global level should shed light on the use of this modification to regulate metabolism, signal transduction, and other processes. We have begun a proteomic analysis of phosphorylation using two-dimensional gel electrophoresis. Chinese hamster ovary ... More
Molecular characterization of Saccharomyces cerevisiae TFIID.
AuthorsSanders SL, Garbett KA, Weil PA
JournalMol Cell Biol
PubMed ID12138208
'We previously defined Saccharomyces cerevisiae TFIID as a 15-subunit complex comprised of the TATA binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). In this report we give a detailed biochemical characterization of this general transcription factor. We have shown that yeast TFIID efficiently mediates both basal and activator-dependent transcription ... More
Quantitative evaluation of proteins in one- and two-dimensional polyacrylamide gels using a fluorescent stain.
AuthorsNishihara JC, Champion KM
JournalElectrophoresis
PubMed ID12210224
'The characteristics of protein detection and quantitation with SYPRO Ruby protein gel stain in one- and two-dimensional polyacrylamide gels were evaluated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of three different purified recombinant proteins showed that the limits of detection were comparable to the limits of detection with ammoniacal silver ... More
Identification of cyclic AMP-regulated genes in Mycobacterium tuberculosis complex bacteria under low-oxygen conditions.
AuthorsGazdik MA, McDonough KA
JournalJ Bacteriol
PubMed ID15805514
'Mycobacterium tuberculosis is the etiological agent of tuberculosis (TB), which kills approximately 2 million people a year despite current treatment options. A greater understanding of the biology of this bacterium is needed to better combat TB disease. The M. tuberculosis genome encodes as many as 15 adenylate cyclases, suggesting that ... More
Proteomics of protein secretion by Bacillus subtilis: separating the "secrets" of the secretome.
AuthorsTjalsma H, Antelmann H, Jongbloed JD, Braun PG, Darmon E, Dorenbos R, Dubois JY, Westers H, Zanen G, Quax WJ, Kuipers OP, Bron S, Hecker M, van Dijl JM
JournalMicrobiol Mol Biol Rev
PubMed ID15187182
'Secretory proteins perform a variety of important "remote-control" functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make predictions about the composition of bacterial machinery for protein secretion as well as the extracellular complement of bacterial proteomes. Recently, the power of proteomics ... More
Constitutive and induced CD44 shedding by ADAM-like proteases and membrane-type 1 matrix metalloproteinase.
AuthorsNakamura H, Suenaga N, Taniwaki K, Matsuki H, Yonezawa K, Fujii M, Okada Y, Seiki M
JournalCancer Res
PubMed ID14871815
'CD44 is a receptor for hyaluronan and mediates signaling that regulates complex cell behavior including cancer cell migration and invasion. Shedding of the extracellular portion of CD44 is the last step in the regulation of the molecule-releasing interaction between the ligand and cell. However, highly glycosylated forms of CD44 have ... More
Comparison of three different fluorescent visualization strategies for detecting Escherichia coli ATP synthase subunits after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
AuthorsBerggren KN, Chernokalskaya E, Lopez MF, Beechem JM, Patton WF
JournalProteomics
PubMed ID11680898
'The correlation between protein molecular weight and the number of lysine or basic amino acid residues was found to be high for broad range molecular weight standards, subunits of Escherichia coli F1F0-ATP synthase and the translated open reading frame of E. coli. A relatively poor correlation between protein molecular weight ... More
Membrane localization of motility, signaling, and polyketide synthetase proteins in Myxococcus xanthus.
AuthorsSimunovic V, Gherardini FC, Shimkets LJ
JournalJ Bacteriol
PubMed ID12923079
'Myxococcus xanthus cells coordinate cellular motility, biofilm formation, and development through the use of cell signaling pathways. In an effort to understand the mechanisms underlying these processes, the inner membrane (IM) and outer membrane (OM) of strain DK1622 were fractionated to examine protein localization. Membranes were enriched from spheroplasts of ... More
Proteomic analysis of S-nitrosylated proteins in mesangial cells.
AuthorsKuncewicz T, Sheta EA, Goldknopf IL, Kone BC
JournalMol Cell Proteomics
PubMed ID12671063
'NO participates in numerous biological events in a variety of cell types including activated glomerular mesangial cells. Many of these events appear to be independent of the known effects of NO on soluble guanylyl cyclase. NO derived from all major isoforms of NO synthase can S-nitrosylate cysteine residues in target ... More
Insect resistance to Bacillus thuringiensis: alterations in the indianmeal moth larval gut proteome.
AuthorsCandas M, Loseva O, Oppert B, Kosaraju P, Bulla LA
JournalMol Cell Proteomics
PubMed ID12601079
'Insect resistance to the Cry toxins of Bacillus thuringiensis (Bt) has been examined previously using a number of traditional biochemical and molecular techniques. In this study, we utilized a proteomic approach involving two-dimensional differential gel electrophoresis, mass spectrometry, and function-based activity profiling to examine changes in the gut proteins from ... More
Proteome analysis reveals elevated serum levels of clusterin in patients with preeclampsia.
AuthorsWatanabe H, Hamada H, Yamada N, Sohda S, Yamakawa-Kobayashi K, Yoshikawa H, Arinami T
JournalProteomics
PubMed ID14760726
'Preeclampsia is a pregnancy-specific syndrome and a major cause of maternal mortality. The pathophysiology of preeclampsia is unknown, and no proteome analysis of preeclampsia has been reported. We sought to identify proteins associated with preeclampsia using a proteomic technique and performed two-dimensional electrophoresis (2-DE) on sera from six patients with ... More
A novel protein tightly bound to bacterial magnetic particles in Magnetospirillum magneticum strain AMB-1.
AuthorsArakaki A, Webb J, Matsunaga T
JournalJ Biol Chem
PubMed ID12496282
'Magnetic bacteria synthesize magnetite crystals with species-dependent morphologies. The molecular mechanisms that control nano-sized magnetite crystal formation and the generation of diverse morphologies are not well understood. From the analysis of magnetite crystal-associated proteins, several low molecular mass proteins tightly bound to bacterial magnetite were obtained from Magnetospirillum magneticum strain ... More
Analysis of N-acetylglucosamine metabolism in the marine bacterium Pirellula sp. strain 1 by a proteomic approach.
AuthorsRabus R, Gade D, Helbig R, Bauer M, Glöckner FO, Kube M, Schlesner H, Reinhardt R, Amann R
JournalProteomics
PubMed ID12112844
'Pirellula sp. strain 1 is a marine bacterium that can grow with the chitin monomer N-acetylglucosamine as sole source of carbon and nitrogen under aerobic conditions, and that is a member of the bacterial phylum Planctomycetes. As a basis for the proteomic studies we quantified growth of strain 1 with ... More
An immediate response of ribosomal transcription to growth factor stimulation in mammals is mediated by ERK phosphorylation of UBF.
'Ribosomal transcription in mammals is regulated in response to growth, differentiation, disease, and aging, but the mechanisms of this regulation have remained unresolved. We show that epidermal growth factor induces immediate, ERK1/2-dependent activation of endogenous ribosomal transcription, while inactivation of ERK1/2 causes an equally immediate reversion to the basal transcription ... More
Poly(ADP-ribosyl)ation as a DNA damage-induced post-translational modification regulating poly(ADP-ribose) polymerase-1-topoisomerase I interaction.
AuthorsYung TM, Sato S, Satoh MS
JournalJ Biol Chem
PubMed ID15247263
'Poly(ADP-ribosyl)ation is a post-translational modification that occurs immediately after exposure of cells to DNA damaging agents. In vivo, 90% of ADP-ribose polymers are attached to the automodification domain of poly(ADP-ribose) polymerase-1 (PARP-1), the main enzyme catalyzing this modification reaction. This enzyme forms complexes with transcription initiation, DNA replication, and DNA ... More
Identification of the SRC pyrimidine-binding protein (SPy) as hnRNP K: implications in the regulation of SRC1A transcription.
AuthorsRitchie SA, Pasha MK, Batten DJ, Sharma RK, Olson DJ, Ross AR, Bonham K
JournalNucleic Acids Res
PubMed ID12595559
'The human SRC gene encodes pp60(c-src), a non-receptor tyrosine kinase involved in numerous signaling pathways. Activation or overexpression of c-Src has also been linked to a number of important human cancers. Transcription of the SRC gene is complex and regulated by two closely linked but highly dissimilar promoters, each associated ... More
A novel saponin hydrolase from Neocosmospora vasinfecta var. vasinfecta.
AuthorsWatanabe M, Sumida N, Yanai K, Murakami T
JournalAppl Environ Microbiol
PubMed ID14766566
'We isolated a soybean saponin hydrolase from Neocosmospora vasinfecta var. vasinfecta PF1225, a filamentous fungus that can degrade soybean saponin and generate soyasapogenol B. This enzyme was found to be a monomer with a molecular mass of about 77 kDa and a glycoprotein. Nucleotide sequence analysis of the corresponding gene ... More
The squamous cell carcinoma antigen 2 inhibits the cysteine proteinase activity of a major mite allergen, Der p 1.
AuthorsSakata Y, Arima K, Takai T, Sakurai W, Masumoto K, Yuyama N, Suminami Y, Kishi F, Yamashita T, Kato T, Ogawa H, Fujimoto K, Matsuo Y, Sugita Y, Izuhara K
JournalJ Biol Chem
PubMed ID14630915
'The squamous cell carcinoma antigens 1 (SCCA1) and SCCA2 belong to the ovalbumin-serpin family. Although SCCA1 and SCCA2 are closely homologous, these two molecules have distinct properties; SCCA1 inhibits cysteine proteinases such as cathepsin K, L, and S, whereas SCCA2 inhibits serine proteinases such as cathepsin G and human mast ... More
One-trial in vitro conditioning regulates a cytoskeletal-related protein (CSP24) in the conditioned stimulus pathway of Hermissenda.
AuthorsCrow T, Xue-Bian JJ
JournalJ Neurosci
PubMed ID12486141
'Hermissenda CSP24 (cytoskeletal-related protein 24) is a 24 kDa beta-thymosin-like protein that is associated with intermediate memory. We showed previously that one-trial conditioning resulted in a significant increase in the phosphorylation of CSP24 detected in lysates of the pathway supporting the conditioned stimulus (CS). Here we report the association of ... More
Nucleophosmin/B23 is a candidate substrate for the BRCA1-BARD1 ubiquitin ligase.
AuthorsSato K, Hayami R, Wu W, Nishikawa T, Nishikawa H, Okuda Y, Ogata H, Fukuda M, Ohta T
JournalJ Biol Chem
PubMed ID15184379
'The breast and ovarian tumor suppressor BRCA1 forms a heterodimeric RING-type ubiquitin ligase with BARD1 to catalyze untraditional Lys-6-linked polyubiquitin chains. It is not clear how the BRCA1-BARD1 ligase regulates various cellular processes such as DNA repair, cell-cycle progression, transcriptional regulation, and centrosome duplication. Here we report that BRCA1-BARD1 catalyzes ... More
Thiourea enhances mapping of the proteome from murine white adipose tissue.
AuthorsLanne B, Potthast F, Höglund A, Brockenhuus von Löwenhielm H, Nyström AC, Nilsson F, Dahllöf B
JournalProteomics
PubMed ID11503206
'Adipose tissue imposes problems in two-dimensional (2-D) analysis due to its extremely high content of fat. To improve protein separation detergents and chaotropes were varied in the IEF step. The most important factor for obtaining distinct spots in the 2-D gel was whether thiourea was included or not. Many high ... More
Bacillus subtilis during feast and famine: visualization of the overall regulation of protein synthesis during glucose starvation by proteome analysis.
AuthorsBernhardt J, Weibezahn J, Scharf C, Hecker M
JournalGenome Res
PubMed ID12566400
'Dual channel imaging and warping of two-dimensional (2D) protein gels were used to visualize global changes of the gene expression patterns in growing Bacillus subtilis cells during entry into the stationary phase as triggered by glucose exhaustion. The 2D gels only depict single moments during the cells'' growth cycle, but ... More
Mobilization of adenine nucleotide translocators as molecular bases of the biochemical threshold effect observed in mitochondrial diseases.
AuthorsFaustin B, Rossignol R, Rocher C, Bénard G, Malgat M, Letellier T
JournalJ Biol Chem
PubMed ID14976187
'The existence of a biochemical threshold effect in the metabolic expression of oxidative phosphorylation deficiencies has considerable implications for the understanding of mitochondrial bioenergetics and the study of mitochondrial diseases. However, the molecular bases of this phenomenon remain unclear. We report here a new mechanism to explain this threshold effect, ... More
Altered gingipain maturation in vimA- and vimE-defective isogenic mutants of Porphyromonas gingivalis.
AuthorsVanterpool E, Roy F, Sandberg L, Fletcher HM
JournalInfect Immun
PubMed ID15731033
'We have previously shown that gingipain activity in Porphyromonas gingivalis is modulated by the unique vimA and vimE genes. To determine if these genes had a similar phenotypic effect on protease maturation and activation, isogenic mutants defective in those genes were further characterized. Western blot analyses with antigingipain antibodies showed ... More
Characterization of the interaction between the J-protein Jac1p and the scaffold for Fe-S cluster biogenesis, Isu1p.
'Jac1p is a conserved, specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria of the yeast Saccharomyces cerevisiae. Although Jac1p as well as its specialized Hsp70 partner, Ssq1p, binds directly to the Fe-S cluster scaffold protein Isu, the Jac1p-Isu1p interaction is not well understood. Here we report ... More
Preferential transcription of rabbit Aldh1a1 in the cornea: implication of hypoxia-related pathways.
AuthorsHough RB, Piatigorsky J
JournalMol Cell Biol
PubMed ID14729976
'Here we examine the molecular basis for the known preferential expression of rabbit aldehyde dehydrogenase class 1 (ALDH1A1) in the cornea. The rabbit Aldh1a1 promoter-firefly luciferase reporter transgene (-3519 to +43) was expressed preferentially in corneal cells in transfection tests and in transgenic mice, with an expression pattern resembling that ... More
A proteomic study of Methylobacterium extorquens reveals a response regulator essential for epiphytic growth.
AuthorsGourion B, Rossignol M, Vorholt JA
JournalProc Natl Acad Sci U S A
PubMed ID16926146
'Aerial plant surfaces are colonized by diverse bacteria such as the ubiquitous Methylobacterium spp. The specific physiological traits as well as the underlying regulatory mechanisms for bacterial plant colonization are largely unknown. The purpose of this study was to identify proteins produced specifically in the phyllosphere by comparing the proteome ... More
Inhibitory mechanism of a cross-class serpin, the squamous cell carcinoma antigen 1.
AuthorsMasumoto K, Sakata Y, Arima K, Nakao I, Izuhara K
JournalJ Biol Chem
PubMed ID12949073
'The squamous cell carcinoma antigen (SCCA) 1 and its homologous molecule, SCCA2, belong to the ovalbumin-serpin family. Although SCCA2 inhibits serine proteinases such as cathepsin G and mast cell chymase, SCCA1 targets cysteine proteinases such as cathepsin S, K, L, and papain. SCCA1 is therefore called a cross-class serpin. The ... More
Practical aspects of fluorescent staining for proteomic applications.
'SYPRO Orange and SYPRO Ruby staining methods, modified for use with large-format two dimensional (2-D) gels, are compared to the manufacturer''s recommended protocols to determine sensitivity and reproducibility of the new methods. This study examines the critical aspects of fixation, washing, and staining to develop an optimized fluorescent staining method. ... More
Huntingtin phosphorylation sites mapped by mass spectrometry. Modulation of cleavage and toxicity.
'Huntingtin (Htt) is a large protein of 3144 amino acids, whose function and regulation have not been well defined. Polyglutamine (polyQ) expansion in the N terminus of Htt causes the neurodegenerative disorder Huntington disease (HD). The cytotoxicity of mutant Htt is modulated by proteolytic cleavage with caspases and calpains generating ... More
A sensitive method for determining the phosphorylation status of natriuretic peptide receptors: cGK-Ialpha does not regulate NPR-A.
AuthorsBryan PM, Smirnov D, Smolenski A, Feil S, Feil R, Hofmann F, Lohmann S, Potter LR
JournalBiochemistry
PubMed ID16430226
'Natriuretic peptide receptor A (NPR-A) and natriuretic peptide receptor B (NPR-B) are transmembrane guanylyl cyclases that catalyze the synthesis of cGMP in response to natriuretic peptides. Phosphorylation and dephosphorylation regulate these receptors and have been traditionally studied by (32)PO(4) labeling of transfected cells. However, this approach cannot be used to ... More
Mutations of bacteriophage T4 59 helicase loader defective in binding fork DNA and in interactions with T4 32 single-stranded DNA-binding protein.
AuthorsJones CE, Green EM, Stephens JA, Mueser TC, Nossal NG
JournalJ Biol Chem
PubMed ID15084598
'Bacteriophage T4 gene 59 protein greatly stimulates the loading of the T4 gene 41 helicase in vitro and is required for recombination and recombination-dependent DNA replication in vivo. 59 protein binds preferentially to forked DNA and interacts directly with the T4 41 helicase and gene 32 single-stranded DNA-binding protein. The ... More
ATP-dependent interactions between Escherichia coli Min proteins and the phospholipid membrane in vitro.
AuthorsLackner LL, Raskin DM, de Boer PA
JournalJ Bacteriol
PubMed ID12533449
'Proper placement of the division apparatus in Escherichia coli requires pole-to-pole oscillation of the MinC division inhibitor. MinC dynamics involves a membrane association-dissociation cycle that is driven by the activities of the MinD ATPase and the MinE topological specificity factor, which themselves undergo coupled oscillatory localization cycles. To understand the ... More
Proteomic analysis reveals alterations in the renal kallikrein pathway during hypoxia-induced hypertension.
AuthorsThongboonkerd V, Gozal E, Sachleben LR, Arthur JM, Pierce WM, Cai J, Chao J, Bader M, Pesquero JB, Gozal D, Klein JB
JournalJ Biol Chem
PubMed ID12121987
'Obstructive sleep apnea syndrome (OSAS), a disorder characterized by episodic hypoxia (EH) during sleep, is associated with systemic hypertension. We used proteomic analysis to examine differences in rat kidney protein expression during EH, and their potential relationship to EH-induced hypertension. Young male Sprague-Dawley rats were exposed to either EH or ... More
Spatial contacts and nucleosome step movements induced by the NURF chromatin remodeling complex.
AuthorsSchwanbeck R, Xiao H, Wu C
JournalJ Biol Chem
PubMed ID15262970
'The nucleosome remodeling factor NURF is a four-subunit, ISWI-containing chromatin remodeling complex that catalyzes nucleosome sliding in an ATP-dependent fashion, thereby modulating the accessibility of the DNA. To elucidate the mechanism of nucleosome sliding, we have investigated by hydroxyl radical footprinting how NURF makes initial contact with a nucleosome positioned ... More
Glycoproteomics of paclitaxel resistance in human epithelial ovarian cancer cell lines: towards the identification of putative biomarkers.
AuthorsDi Michele M, Marcone S, Cicchillitti L, Della Corte A, Ferlini C, Scambia G, Donati MB, Rotilio D,
JournalJ Proteomics
PubMed ID19951750
'Glycosylation, one of the most common post translational modifications (PTMs) of proteins, is often associated with carcinogenesis and tumor malignancy. Ovarian cancer is the sixth cause of cancer-related death in Western countries. Currently, it is treated by debulking surgery followed by chemotherapy based on paclitaxel, alone or in combination with ... More
Importance of different tfd genes for degradation of chloroaromatics by Ralstonia eutropha JMP134.
AuthorsPlumeier I, Pérez-Pantoja D, Heim S, González B, Pieper DH
JournalJ Bacteriol
PubMed ID12107121
'The tfdC(I)D(I)E(I)F(I,) and tfdD(II)C(II)E(II)F(II) gene modules of plasmid pJP4 of Ralstonia eutropha JMP134 encode complete sets of functional enzymes for the transformation of chlorocatechols into 3-oxoadipate, which are all expressed during growth on 2,4-dichlorophenoxyacetate (2,4-D). However, activity of tfd(I)-encoded enzymes was usually higher than that of tfd(II)-encoded enzymes, both in ... More
Effect of adaptation to ethanol on cytoplasmic and membrane protein profiles of Oenococcus oeni.
AuthorsSilveira MG, Baumgärtner M, Rombouts FM, Abee T
JournalAppl Environ Microbiol
PubMed ID15128528
'The practical application of commercial malolactic starter cultures of Oenococcus oeni surviving direct inoculation in wine requires insight into mechanisms of ethanol toxicity and of acquired ethanol tolerance in this organism. Therefore, the site-specific location of proteins involved in ethanol adaptation, including cytoplasmic, membrane-associated, and integral membrane proteins, was investigated. ... More
Partial structure of glutamic acid and alanine-rich protein, a major surface glycoprotein of the insect stages of Trypanosoma congolense.
AuthorsThomson LM, Lamont DJ, Mehlert A, Barry JD, Ferguson MA
JournalJ Biol Chem
PubMed ID12368279
'The tsetse fly transmitted salivarian trypanosome, Trypanosoma congolense of the subgenus Nanomonas, is the most significant of the trypanosomes with respect to the pathology of livestock in sub-Saharan Africa. Unlike the related trypanosome Trypanosoma brucei of the subgenus Trypanozoon, the major surface molecules of the insect stages of T. congolense ... More
Topography of the euryarchaeal transcription initiation complex.
AuthorsBartlett MS, Thomm M, Geiduschek EP
JournalJ Biol Chem
PubMed ID14617625
'Transcription in the Archaea is carried out by RNA polymerases and transcription factors that are highly homologous to their eukaryotic counterparts, but little is known about the structural organization of the archaeal transcription complex. To address this, transcription initiation complexes have been formed with Pyrococcus furiosus transcription factors (TBP and ... More
Structure-function analysis of PrsA reveals roles for the parvulin-like and flanking N- and C-terminal domains in protein folding and secretion in Bacillus subtilis.
AuthorsVitikainen M, Lappalainen I, Seppala R, Antelmann H, Boer H, Taira S, Savilahti H, Hecker M, Vihinen M, Sarvas M, Kontinen VP
JournalJ Biol Chem
PubMed ID14976191
'The PrsA protein of Bacillus subtilis is an essential membrane-bound lipoprotein that is assumed to assist post-translocational folding of exported proteins and stabilize them in the compartment between the cytoplasmic membrane and cell wall. This folding activity is consistent with the homology of a segment of PrsA with parvulin-type peptidyl-prolyl ... More
Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis.
AuthorsSingh S, Powell DW, Rane MJ, Millard TH, Trent JO, Pierce WM, Klein JB, Machesky LM, McLeish KR
JournalJ Biol Chem
PubMed ID12829704
'The p38 MAPK pathway regulates multiple neutrophil functional responses via activation of the serine-threonine kinase MAPK-activated protein kinase 2 (MAPKAPK2). To identify substrates of MAPKAPK2 that mediate these responses, a proteomic approach was used in which in vitro phosphorylation of neutrophil lysates by exogenously added active recombinant MAPKAPK2 was followed ... More
Selective contribution of the twin-arginine translocation pathway to protein secretion in Bacillus subtilis.
AuthorsJongbloed JD, Antelmann H, Hecker M, Nijland R, Bron S, Airaksinen U, Pries F, Quax WJ, van Dijl JM, Braun PG
JournalJ Biol Chem
PubMed ID12218047
'The availability of the complete genome sequence of Bacillus subtilis has allowed the prediction of all exported proteins of this Gram-positive eubacterium. Recently, approximately 180 secretory and 114 lipoprotein signal peptides were predicted to direct protein export from the cytoplasm. Whereas most exported proteins appear to use the Sec pathway, ... More
An improved formulation of SYPRO Ruby protein gel stain: comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation.
AuthorsBerggren KN, Schulenberg B, Lopez MF, Steinberg TH, Bogdanova A, Smejkal G, Wang A, Patton WF
JournalProteomics
PubMed ID11987123
'SYPRO Ruby protein gel stain is compatible with a variety of imaging platforms since it absorbs maximally in the ultraviolet (280 nm) and visible (470 nm) regions of the spectrum. Dye localization is achieved by noncovalent, electrostatic and hydrophobic binding to proteins, with signal being detected at 610 nm. Since ... More
Protein expression changes in spinal muscular atrophy revealed with a novel antibody array technology.
AuthorsAnderson K, Potter A, Baban D, Davies KE
JournalBrain
PubMed ID12847076
'Autosomal recessive proximal spinal muscular atrophy (SMA) is a severe neurodegenerative disease of childhood causing weakness and wasting secondary to motor neuron dysfunction. Over 97% of cases are caused by deletions or mutations within the survival motor neuron (SMN) gene. The SMN protein is highly expressed within brain, spinal cord ... More
CXCR4 heterogeneity in primary cells: possible role of ubiquitination.
AuthorsLapham CK, Romantseva T, Petricoin E, King LR, Manischewitz J, Zaitseva MB, Golding H
JournalJ Leukoc Biol
PubMed ID12488503
'The chemokine receptor CXCR4 is a primary coreceptor for the HIV-1 virus. The predicted molecular weight (MW) of glycosylated CXCR4 is 45-47 kDa. However, immunoblots of whole cell lysates from human lymphocytes, monocytes, macrophages, and the Jurkat T-lymphocyte line revealed multiple MW isoforms of CXCR4. Three of the bands could ... More
Two-dimensional gel proteome reference map of blood monocytes.
AuthorsJin M, Diaz PT, Bourgeois T, Eng C, Marsh CB, Wu HM
JournalProteome Sci
PubMed ID16948843
'BACKGROUND: Blood monocytes play a central role in regulating host inflammatory processes through chemotaxis, phagocytosis, and cytokine production. However, the molecular details underlying these diverse functions are not completely understood. Understanding the proteomes of blood monocytes will provide new insights into their biological role in health and diseases. RESULTS: In ... More
The effect of RNA interference Down-regulation of RNA editing 3'-terminal uridylyl transferase (TUTase) 1 on mitochondrial de novo protein synthesis and stability of respiratory complexes in Trypanosoma brucei.
AuthorsNebohácová M, Maslov DA, Falick AM, Simpson L
JournalJ Biol Chem
PubMed ID14681226
'Inhibition of RNA editing by down-regulation of expression of the mitochondrial RNA editing TUTase 1 by RNA interference had profound effects on kinetoplast biogenesis in Trypanosoma brucei procyclic cells. De novo synthesis of the apocytochrome b and cytochrome oxidase subunit I proteins was no longer detectable after 3 days of ... More
Imaging technologies for the detection of multiple stains in proteomics.
AuthorsMiura K
JournalProteomics
PubMed ID12872211
'Laser-based scanners and charge-coupled device (CCD) camera systems are evolving to have greater functional capabilities for capturing images from a range of staining technologies used in gel electrophoresis and electroblotting. Digitizing Coomassie Brilliant Blue (CBB) stained gels and silver stained gels has now become possible using a laser-based gel scanner, ... More
'Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, causes human monocyte/macrophage-tropic ehrlichiosis. In this study, proteomic approaches were used to demonstrate host cell-specific antigenic expression by E. chaffeensis. The differentially expressed antigens include those from the 28-kDa outer membrane protein (p28-Omp) multigene locus. The proteins expressed in infected macrophages are the products ... More
Inhibition of SAPK2a/p38 prevents hnRNP A0 phosphorylation by MAPKAP-K2 and its interaction with cytokine mRNAs.
AuthorsRousseau S, Morrice N, Peggie M, Campbell DG, Gaestel M, Cohen P
JournalEMBO J
PubMed ID12456657
'Lipopolysaccharide (LPS) stimulates production of inflammatory mediators, partly by stabilizing [interleukin-6 (IL-6), cyclooxygenase 2 (COX-2)] and/or stimulating translation [tumour necrosis factor-alpha (TNF-alpha)] of their mRNAs. Such regulation depends on AU-rich elements (AREs) within the 3''-untranslated regions and is partially suppressed by SB 203580 (which inhibits SAPK2a/p38). The LPS-induced production of ... More
Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells.
'Comparative analysis of cytoplasmic organelles in a variety of tumors relative to normal tissues generally reveals a strong diminution in mitochondrial content and in oxidative phosphorylation capacity. However, little is known about what triggers these modifications and whether or not they are physiologically reversible. We hypothesized that energy substrate availability ... More
Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease.
AuthorsPuchades M, Hansson SF, Nilsson CL, Andreasen N, Blennow K, Davidsson P
JournalBrain Res Mol Brain Res
PubMed ID14559363
'By comparing the cerebrospinal fluid (CSF) proteome between Alzheimer''s disease (AD) patients and controls, it may be possible to identify proteins that play a role in the disease process and thus to study the pathogenesis of AD. Two-dimensional gel electrophoresis (2-DE), SYPRO Ruby staining and mass spectrometry were used for ... More
BfpU, a soluble protein essential for type IV pilus biogenesis in enteropathogenic Escherichia coli.
AuthorsSchreiber W, Stone KD, Strong MA, DeTolla LJ, Hoppert M, Donnenberg MS
JournalMicrobiology
PubMed ID12177344
'A cluster of 14 genes located on the large plasmid of enteropathogenic Escherichia coli (EPEC) strains is sufficient to direct the biogenesis of the type IV bundle-forming pilus (BFP) in a recombinant E. coli host. The fifth gene in the cluster, bfpU, encodes a protein that is predicted to be ... More
Mass spectrometric and mutational analyses reveal Lys-6-linked polyubiquitin chains catalyzed by BRCA1-BARD1 ubiquitin ligase.
AuthorsNishikawa H, Ooka S, Sato K, Arima K, Okamoto J, Klevit RE, Fukuda M, Ohta T
JournalJ Biol Chem
PubMed ID14638690
'The breast and ovarian cancer suppressor BRCA1 acquires significant ubiquitin ligase activity when bound to BARD1 as a RING heterodimer. Although the activity may well be important for the role of BRCA1 as a tumor suppressor, the biochemical consequence of the activity is not yet known. Here we report that ... More
Blarina toxin, a mammalian lethal venom from the short-tailed shrew Blarina brevicauda: Isolation and characterization.
AuthorsKita M, Nakamura Y, Okumura Y, Ohdachi SD, Oba Y, Yoshikuni M, Kido H, Uemura D
JournalProc Natl Acad Sci U S A
PubMed ID15136743
'Venomous mammals are rare, and their venoms have not been characterized. We have purified and characterized the blarina toxin (BLTX), a lethal mammalian venom with a tissue kallikrein-like activity from the submaxillary and sublingual glands of the short-tailed shrew Blarina brevicauda. Mice administered BLTX i.p. developed irregular respiration, paralysis, and ... More
Detection technologies in proteome analysis.
AuthorsPatton WF
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID12015990
'Common strategies employed for general protein detection include organic dye, silver stain, radiolabeling, reverse stain, fluorescent stain, chemiluminescent stain and mass spectrometry-based approaches. Fluorescence-based protein detection methods have recently surpassed conventional technologies such as colloidal Coomassie blue and silver staining in terms of quantitative accuracy, detection sensitivity, and compatibility with ... More
Isolation protocol for two-dimensional-polyacrylamide gel electrophoresis analysis of Haloferax volcanii proteome.
Protein stains for proteomic applications: which, when, why?
AuthorsMiller I, Crawford J, Gianazza E,
JournalProteomics
PubMed ID16991193
'This review recollects literature data on sensitivity and dynamic range for the most commonly used colorimetric and fluorescent dyes for general protein staining, and summarizes procedures for the most common PTM-specific detection methods. It also compiles some important points to be considered in imaging and evaluation. In addition to theoretical ... More
Optimized conditions for diluting and reusing a fluorescent protein gel stain.
AuthorsAhnert N, Patton WF, Schulenberg B
JournalElectrophoresis
PubMed ID15300769
'This study elucidates the optimum conditions at the minimum cost for using SYPRO Ruby protein gel stain. It deals with the effects of gel fixation and staining times, as well as dilution and reuse of SYPRO Ruby protein gel stain in one-dimensional (1-D) gels. Signal strength and dynamic range were ... More
TIP120A associates with cullins and modulates ubiquitin ligase activity.
AuthorsMin KW, Hwang JW, Lee JS, Park Y, Tamura TA, Yoon JB
JournalJ Biol Chem
PubMed ID12609982
'The cullin-containing ubiquitin-protein isopeptide ligases (E3s) play an important role in regulating the abundance of key proteins involved in cellular processes such as cell cycle and cytokine signaling. They have multisubunit modular structures in which substrate recognition and the catalysis of ubiquitination are carried out by distinct polypeptides. In a ... More
A dominant-negative MEC3 mutant uncovers new functions for the Rad17 complex and Tel1.
AuthorsGiannattasio M, Sommariva E, Vercillo R, Lippi-Boncambi F, Liberi G, Foiani M, Plevani P, Muzi-Falconi M
JournalProc Natl Acad Sci U S A
PubMed ID12271137
'The Rad17-Mec3-Ddc1 complex is essential for the cellular response to genotoxic agents and is thought to be important for sensing DNA lesions. Deletion of any of the RAD17, MEC3 or DDC1 genes abolishes the G(1) and G(2) and impairs the intra-S DNA-damage checkpoints. We characterize a dominant-negative mec3-dn mutation that ... More
The ferroxidase activity of yeast frataxin.
AuthorsPark S, Gakh O, Mooney SM, Isaya G
JournalJ Biol Chem
PubMed ID12149269
'Frataxin is required for maintenance of normal mitochondrial iron levels and respiration. The mature form of yeast frataxin (mYfh1p) assembles stepwise into a multimer of 840 kDa (alpha(48)) that accumulates iron in a water-soluble form. Here, two distinct iron oxidation reactions are shown to take place during the initial assembly ... More
Aquifex aeolicus PilT, homologue of a surface motility protein, is a thermostable oligomeric NTPase.
AuthorsHerdendorf TJ, McCaslin DR, Forest KT
JournalJ Bacteriol
PubMed ID12426333
'Bacterial surface motility works by retraction of surface-attached type IV pili. This retraction requires the PilT protein, a member of a large family of putative NTPases from type II and IV secretion systems. In this study, the PilT homologue from the thermophilic eubacterium Aquifex aeolicus was cloned, overexpressed, and purified. ... More
The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p.
AuthorsSmotrys JE, Schoenfish MJ, Stutz MA, Linder ME
JournalJ Cell Biol
PubMed ID16186255
'Palmitoylation of the vacuolar membrane protein Vac8p is essential for vacuole fusion in yeast (Veit, M., R. Laage, L. Dietrich, L. Wang, and C. Ungermann. 2001. EMBO J. 20:3145-3155; Wang, Y.X., E.J. Kauffman, J.E. Duex, and L.S. Weisman. 2001. J. Biol. Chem. 276:35133-35140). Proteins that contain an Asp-His-His-Cys (DHHC)-cysteine rich ... More
Discovery of a fusion kinase in EOL-1 cells and idiopathic hypereosinophilic syndrome.
AuthorsGriffin JH, Leung J, Bruner RJ, Caligiuri MA, Briesewitz R
JournalProc Natl Acad Sci U S A
PubMed ID12808148
'Idiopathic hypereosinophilic syndrome (HES) is a myeloproliferative disease of unknown etiology. Recently, it has been reported that imatinib mesylate (Gleevec), an inhibitor of Bcr-Abl kinase useful in the treatment of chronic myeloid leukemia, is also effective in treating HES; however, the molecular target of imatinib in HES is unknown. This ... More
Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast: regulating adaptation to citric acid stress.
'Screening the Saccharomyces cerevisiae disruptome, profiling transcripts, and determining changes in protein expression have identified an important new role for the high-osmolarity glycerol (HOG) mitogen-activated protein kinase (MAPK) pathway in the regulation of adaptation to citric acid stress. Deletion of HOG1, SSK1, PBS2, PTC2, PTP2, and PTP3 resulted in sensitivity ... More
Isolation of rodent airway epithelial cell proteins facilitates in vivo proteomics studies of lung toxicity.
'Recent developments in genomics, proteomics, and metabolomics hold substantial promise for understanding cellular responses to toxicants. Gene expression profiling is now considered standard procedure, but numerous publications reporting a lack of correlation between mRNA and protein expression emphasize the importance of conducting parallel proteomics studies. The cellular complexity of the ... More
Evaluation of saturation labelling two-dimensional difference gel electrophoresis fluorescent dyes.
AuthorsShaw J, Rowlinson R, Nickson J, Stone T, Sweet A, Williams K, Tonge R
JournalProteomics
PubMed ID12872219
'Two-dimensional difference gel electrophoresis (2-D DIGE) enables an increased confidence in detection of protein differences. However, due to the nature of the minimal labelling where only approximately 5% of a given protein is labelled, spots cannot be directly excised for mass spectrometry (MS) analysis and detection sensitivity could be further ... More
Clp protease complexes from photosynthetic and non-photosynthetic plastids and mitochondria of plants, their predicted three-dimensional structures, and functional implications.
AuthorsPeltier JB, Ripoll DR, Friso G, Rudella A, Cai Y, Ytterberg J, Giacomelli L, Pillardy J, van Wijk KJ
JournalJ Biol Chem
PubMed ID14593120
'Tetradecameric Clp protease core complexes in non-photosynthetic plastids of roots, flower petals, and in chloroplasts of leaves of Arabidopsis thaliana were purified based on native mass and isoelectric point and identified by mass spectrometry. The stoichiometry between the subunits was determined. The protease complex consisted of one to three copies ... More
Role of insulin action and cell size on protein expression patterns in adipocytes.
'Mice with a fat-specific insulin receptor knock-out (FIRKO) exhibit a polarization of white adipose tissue into two populations of cells, one small (diameter <50 microm) and one large (diameter >100 microm), accompanied by changes in insulin-stimulated glucose uptake, triglyceride synthesis, and lipolysis. To characterize these subclasses of adipocytes, we have ... More
Ian4 is required for mitochondrial integrity and T cell survival.
AuthorsPandarpurkar M, Wilson-Fritch L, Corvera S, Markholst H, Hornum L, Greiner DL, Mordes JP, Rossini AA, Bortell R
JournalProc Natl Acad Sci U S A
PubMed ID12930893
'Apoptosis is a regulated cell death program controlled by extrinsic and intrinsic signaling pathways. The intrinsic pathway involves stress signals that activate pro-apoptotic members of the Bcl-2 family, inducing permeabilization of mitochondria and release of apoptogenic factors. These proteins localize to the outer mitochondrial membrane. Ian4, a mitochondrial outer membrane ... More
A murine model for neuropsychiatric disorders associated with group A beta-hemolytic streptococcal infection.
AuthorsHoffman KL, Hornig M, Yaddanapudi K, Jabado O, Lipkin WI
JournalJ Neurosci
PubMed ID14973249
'A syndrome of motoric and neuropsychiatric symptoms comprising various elements, including chorea, hyperactivity, tics, emotional lability, and obsessive-compulsive symptoms, can occur in association with group A beta-hemolytic streptococcal (GABHS) infection. We tested the hypothesis that an immune response to GABHS can result in behavioral abnormalities. Female SJL/J mice were immunized ... More
Interaction of low molecular weight group IIA phospholipase A2 with apoptotic human T cells: role of heparan sulfate proteoglycans.
AuthorsBoilard E, Bourgoin SG, Bernatchez C, Poubelle PE, Surette ME
JournalFASEB J
PubMed ID12773489
'Human group IIA phospholipase A2 (hIIA PLA2) is a 14 kDa secreted enzyme associated with inflammatory diseases. A newly discovered property of hIIA PLA2 is the binding affinity for the heparan sulfate proteoglycan (HSPG) glypican-1. In this study, the binding of hIIA PLA2 to apoptotic human T cells was investigated. ... More
High-molecular-weight protein (pUL48) of human cytomegalovirus is a competent deubiquitinating protease: mutant viruses altered in its active-site cysteine or histidine are viable.
AuthorsWang J, Loveland AN, Kattenhorn LM, Ploegh HL, Gibson W
JournalJ Virol
PubMed ID16731939
'We show here that the high-molecular-weight protein (HMWP or pUL48; 253 kDa) of human cytomegalovirus (HCMV) is a functionally competent deubiquitinating protease (DUB). By using a suicide substrate probe specific for ubiquitin-binding cysteine proteases (DUB probe) to screen lysates of HCMV-infected cells, we found just one infected-cell-specific DUB. Characteristics of ... More
Disruption of the zinc finger motifs in the Leishmania tarentolae LC-4 (=TbMP63) L-complex editing protein affects the stability of the L-complex.
AuthorsKang X, Falick AM, Nelson RE, Gao G, Rogers K, Aphasizhev R, Simpson L
JournalJ Biol Chem
PubMed ID14604987
'The uridine insertion/deletion editing complex, which we have termed the L-complex, is composed of at least 16 polypeptides stabilized entirely by protein-protein interactions. Three L-complex proteins contain zinc finger motifs that could be involved in these interactions. In Leishmania these proteins are labeled LC-1, LC-4, and LC-7b, and the orthologs ... More
Fluoride exposure attenuates expression of Streptococcus pyogenes virulence factors.
AuthorsThongboonkerd V, Luengpailin J, Cao J, Pierce WM, Cai J, Klein JB, Doyle RJ
JournalJ Biol Chem
PubMed ID11867637
'Fluoridation causes an obvious reduction of dental caries by interference with cariogenic streptococci. However, the effect of fluoride on group A streptococci that causes rheumatic fever and acute poststreptococcal glomerulonephritis is not known. We have used proteomic analysis to create a reference proteome map for Streptococcus pyogenes and to determine ... More
Glucosamine-induced alterations of mitochondrial function in pancreatic beta-cells: possible role of protein glycosylation.
AuthorsAnello M, Spampinato D, Piro S, Purrello F, Rabuazzo AM
JournalAm J Physiol Endocrinol Metab
PubMed ID15149952
'Chronic exposure of rat pancreatic islets and INS-1 insulinoma cells to glucosamine (GlcN) produced a reduction of glucose-induced (22.2 mM) insulin release that was associated with a reduction of ATP levels and ATP/ADP ratio compared with control groups. To further evaluate mitochondrial function and ATP metabolism, we then studied uncoupling ... More
Proteome analysis of vinca alkaloid response and resistance in acute lymphoblastic leukemia reveals novel cytoskeletal alterations.
AuthorsVerrills NM, Walsh BJ, Cobon GS, Hains PG, Kavallaris M
JournalJ Biol Chem
PubMed ID12949081
'Vinca alkaloids are used widely in the treatment of both childhood and adult cancers. Their cellular target is the beta-tubulin subunit of alpha/beta-tubulin heterodimers, and they act to inhibit cell division by disrupting microtubule dynamics. Despite the effectiveness of these agents, drug resistance is a major clinical problem. To identify ... More
Small heat-shock protein Hsp20 phosphorylation inhibits beta-agonist-induced cardiac apoptosis.
AuthorsFan GC, Chu G, Mitton B, Song Q, Yuan Q, Kranias EG
JournalCirc Res
PubMed ID15105294
'Activation of the sympathetic nervous system is a common compensatory feature in heart failure, but sustained beta-adrenergic activation induces cardiomyocyte death, leading to cardiac remodeling and dysfunction. In mouse cardiomyocytes, we recently reported that prolonged exposure to beta-agonists is associated with transient increases in expression and phosphorylation of a small ... More
In vitro serial passage of Staphylococcus aureus: changes in physiology, virulence factor production, and agr nucleotide sequence.
'Recently, we observed that Staphylococcus aureus strains newly isolated from patients had twofold-higher aconitase activity than a strain passaged extensively in vitro, leading us to hypothesize that aconitase specific activity decreases over time during in vitro passage. To test this hypothesis, a strain recovered from a patient with toxic shock ... More
Fhit is a physiological target of the protein kinase Src.
AuthorsPekarsky Y, Garrison PN, Palamarchuk A, Zanesi N, Aqeilan RI, Huebner K, Barnes LD, Croce CM
JournalProc Natl Acad Sci U S A
PubMed ID15007172
'The FHIT gene is a tumor suppressor that is frequently inactivated by genomic alterations at chromosomal region 3p14.2. In the last few years, a considerable amount of data describing inactivation of FHIT in a variety of human malignancies and demonstrating the tumor suppressor potential of Fhit have been reported. Despite ... More
Joining mutants of RAG1 and RAG2 that demonstrate impaired interactions with the coding-end DNA.
AuthorsNagawa F, Hirose S, Nishizumi H, Nishihara T, Sakano H
JournalJ Biol Chem
PubMed ID15249552
'In V(D)J joining of antigen receptor genes, two recombination signal sequences (RSSs), 12- and 23-RSSs, form a complex with the protein products of recombination activating genes, RAG1 and RAG2. DNaseI footprinting demonstrates that the interaction of RAG proteins with substrate RSS DNA is not just limited to the signal region ... More
Fluorescent imaging and analysis with Typhoon 8600.
AuthorsMcNamara P, Lew W, Han L
JournalElectrophoresis
PubMed ID11332750
'Current technologies in laser-based imaging systems for life science applications offer highly sensitive and quantitative detection of multiple labels. Typhoon 8600 variable mode imager is capable of fluorescence, storage phosphor, and chemiluminescence detection of gels and blots. The system delivers sensitive fluorescent detection of nucleic acids and proteins in both ... More
Presence of multiple mRNA cycling sequence element-binding proteins in Crithidia fasciculata.
AuthorsMittra B, Sinha KM, Hines JC, Ray DS
JournalJ Biol Chem
PubMed ID12730192
'A consensus sequence present in the 5''- or 3''-untranslated regions of several Crithidia fasciculata messenger RNAs encoding proteins involved in DNA metabolism has been shown to be necessary for the periodic accumulation of these mRNAs during the cell cycle. A protein complex termed cycling sequence-binding protein (CSBP) has two subunits, ... More
Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana.
AuthorsShimaoka T, Ohnishi M, Sazuka T, Mitsuhashi N, Hara-Nishimura I, Shimazaki K, Maeshima M, Yokota A, Tomizawa K, Mimura T
JournalPlant Cell Physiol
PubMed ID15215502
'A large number of proteins in the tonoplast, including pumps, carriers, ion channels and receptors support the various functions of the plant vacuole. To date, few proteins involved in these activities have been identified at the molecular level. In this study, proteomic analysis was used to identify new tonoplast proteins. ... More
Plasma proteome of severe acute respiratory syndrome analyzed by two-dimensional gel electrophoresis and mass spectrometry.
AuthorsChen JH, Chang YW, Yao CW, Chiueh TS, Huang SC, Chien KY, Chen A, Chang FY, Wong CH, Chen YJ
JournalProc Natl Acad Sci U S A
PubMed ID15572443
'We have investigated the plasma proteome by using 2D gel electrophoresis and MS from patients with severe acute respiratory syndrome (SARS). A complete proteomic analysis was performed on four patients with SARS in different time courses, and a total of 38 differential spots were selected for protein identification. Most of ... More