Invitrogen™

SNARF™-4F 5-(and-6)-Carboxylic Acid

Catalog number:  S23920

Related applications:

Cell Viability, Proliferation & Function

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Description

The cell-impermeant ratiometric pH indicator SNARF®-4F 5-(and-6)-carboxylic acid (pKa of ~6.4) exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader
Cell Permeability: Cell-Impermeant
Detection Method: Fluorescent
Indicator Properties: Fluorescent Dye-Based
Indicator: pH Indicator
Shipping Condition: Room Temperature
Product Line: SNARF®

Contents & storage

Store at room temperature and protect from light.