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SNARF®-5F 5-(and-6)-Carboxylic Acid

Catalog number: S23922

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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Description

The cell-impermeant ratiometric pH indicator SNARF®-5F 5-(and-6)-carboxylic acid (pKa of ~7.2) exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: pH Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Microplate Reader, Flow Cytometer, Fluorescence Microscope
Cell Permeability: Cell-Impermeant
Product Size: 1 mg
Detection Method: Fluorescent
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature and protect from light.

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