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Invitrogen
Rabbit anti-Human IgM Secondary Antibody
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FIGURE: 1 / 7
Human IgM Secondary Antibody (SA1-400) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Human IgM Secondary Antibody was performed using log phase Ramos cells (IgM producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Rabbit anti-Human IgM Secondary Antibody (Product # SA1-400) at 1:2... View More
Product Details
SA1-400
Applications
Tested Dilution
Western Blot (WB)
1:2000
Immunohistochemistry (IHC)
1:50 - 1:100
Immunocytochemistry (ICC/IF)
1:250
ELISA (ELISA)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Purified human IgM Fc 5 mu fragment.
Conjugate
Form
Liquid
Concentration
1.8 mg/mL
Amount
1.5 mg
Purification
Affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgM
Antibody Form
Whole Antibody
Product Specific Information
SA1-400 reacts with human IgM, and does not react with human IgG, IgA, kappa or lambda chains.
SA1-400 has been successfully used in immunohistochemistry, ELISA, and Western blot procedures. Avoid prolonged fixation in buffered formalin as this can destroy the epitope.
The SA1-400 immunogen is purified human IgM Fc 5 mu fragment.
Country of Origin: USA
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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