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Invitrogen
Alpaca anti-Mouse IgG1 Nano (VHH) Recombinant Secondary Antibody, Alexa Fluor™ 488
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FIGURE: 1 / 2
Mouse IgG1 Nano (VHH) Secondary Antibody (SA5-10328) in ICC/IF
Immunofluorescence analysis in HeLa cells. Samples were incubated with Mouse IgG1 anti-Vimentin (grey); Mouse IgG2b anti-Lamin (green); Mouse IgG3 anti-MOT (red) primary antibodies and followed by Mouse IgG1 (VHH) secondary antibody (Product # SA5-10329), alpaca anti-mouse IgG1 VHH Alexa Fluor 647; alpaca anti-mouse IgG2b VHH Alexa Fluor 488; and alpaca anti-mouse IgG3 VHH Alexa Fluor 568. The last image is... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
SA5-10328
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunocytochemistry (ICC/IF)
1:500
Flow Cytometry (Flow)
Assay-dependent
Dot blot (DB)
Assay-dependent
Immunomicroscopy (IM)
1:500
Product Specifications
Host/Isotype
Alpaca
/ IgG1
Expression System
E. coli
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
CTK0103/CTK0104
Immunogen
Purified mouse IgG1
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
5 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG1
Antibody Form
VHH
Product Specific Information
Biclonal nanobodies specific for mouse IgG1 Fc. CTK0103: KD =0.13 nM, CTK0104: KD = 0.63nM
Non Cross-Reactivity includes: Goat, guinea pig, rabbit, rat, sheep, human, and macaque serum and to mouse IgG2a, IgG2b, IgG2c, and IgG3.
Rapid workflow: Simultaneous incubation of mouse IgG1 primary antibody and Alpaca anti-mouse IgG1 Nano (VHH) recombinant secondary antibody.
Offered through a partnership with ChromoTek.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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