The Invitrogen SureLock Tandem Transfer Tray is used to facilitate wet transfer of midi gels using the SureLock Tandem Blot Module
and SureLock Tandem Midi Gel Tank
. The tray has two compartments: the front compartment is used to soak sponge pads and filter papers, and the back compartment is used to position the SureLock Tandem Blot Module to build a transfer stack. The tray has graphics showing the correct orientation for stack assembly and two pour spouts for spill proof pouring of transfer buffer.About the SureLock Tandem Midi Blot Module
The SureLock Tandem Midi Blot Module is a wet transfer device used exclusively with the SureLock Tandem Midi Gel Tank
. The tank accommodates one blot module per chamber, or two blot modules total. The SureLock Tandem Midi Blot Module is compatible with all Invitrogen midi gels (blot size: 8.5 cm x 14 cm). The blot module accommodates one midi gel per wet transfer. The unique design make the module easy to use, while the efficient size of the inner core uses less transfer buffer per transfer (only ∼300 mL per transfer). Since transfer buffer contains methanol, this also results in less hazardous waste requiring special disposal. At the recommended conditions and constant voltage, proteins can be transferred to nitrocellulose or PVDF membranes in 30 minutes and at room temperature.