I am using Click-iT AHA (L-azidohomoalanine) kit to label nascent proteins in live cells, then detecting with TAMRA alkyne after fixation and permeabilization and a click reaction. But I'm seeing nucleolar labeling in the cells. It this expected?
Yes. All proteins synthesized during the time the AHA is present will be detected, and they may be all over the cell. Our imaging shows strong labeling in the nucleoli and cytoplasm, as well as nuclear labeling.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.