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OriGene
USP25 Monoclonal Antibody (OTI6H4), TrueMAB™
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FIGURE: 1 / 1
USP25 Antibody (TA810971S) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP25 (RC209755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-USP25. Positive lysates LY415616 (100 µg) and LC415616 (20 µg) can be purchased separately from OriGene.
Product Details
TA810971S
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI6H4
Immunogen
Full length human recombinant protein of human USP25 produced in HEK293T cell.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
30 µg
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: deubiquitinating enzyme; Deubiquitinating enzyme 25; Ubiquitin carboxyl-terminal hydrolase 25; Ubiquitin thioesterase 25; ubiquitin thiolesterase 25; ubiquitin-specific processing protease 25; Ubiquitin-specific-processing protease 25; unnamed protein product; USP on chromosome 21; USP25m; only found in skeletal muscle and heart
Gene Aliases: EIG19; USP21; USP25
UniProt ID: (Human) Q9UHP3
Entrez Gene ID: (Human) 29761
proteolysis
protein deubiquitination
regulation of protein stability
cellular response to oxidative stress
protein modification process
interleukin-17A-mediated signaling pathway
protein K63-linked deubiquitination
protein K48-linked ubiquitination
protein K48-linked deubiquitination
negative regulation of response to oxidative stress
negative regulation of interleukin-17-mediated signaling pathway
negative regulation of ERAD pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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