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UCHL1 Monoclonal Antibody (UMAB244), UltraMAB™
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FIGURE: 1 / 11
UCHL1 Antibody (UM800136CF) in IHC (P)
Immunohistochemical staining of paraffin-embedded human embryonic brain cortex tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM800136)(1:600)
Product Details
UM800136CF
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
UMAB244
Immunogen
Full length human recombinant protein of human UCHL1 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PGP 9.5; PGP9.5; protein gene product 9.5; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; ubiquitin thiolesterase; UCH-L1; UCHL; unnamed protein product
Gene Aliases: AW822034; C88048; gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; R75593; SPG79; SPG79A; UCH-L1; UCHL-1; UCHL1
UniProt ID: (Human) P09936, (Mouse) Q9R0P9, (Rat) Q00981
Entrez Gene ID: (Human) 7345, (Mouse) 22223, (Rat) 29545
cysteine-type endopeptidase activity
thiol-dependent ubiquitin-specific protease activity
protein binding
peptidase activity
cysteine-type peptidase activity
omega peptidase activity
hydrolase activity
receptor inhibitor activity
ubiquitin protein ligase binding
alpha-2A adrenergic receptor binding
ribosome binding
ubiquitin binding
ligase activity
cysteine protease
male germ cell proliferation
response to ischemia
proteolysis
ubiquitin-dependent protein catabolic process
axonogenesis
axon target recognition
adult walking behavior
regulation of macroautophagy
protein deubiquitination
axonal transport of mitochondrion
protein catabolic process
eating behavior
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of MAPK cascade
positive regulation of glycolytic process
neuromuscular process
muscle cell development
cellular response to xenobiotic stimulus
cell proliferation
sensory perception of pain
negative regulation of MAP kinase activity
muscle fiber development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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